| Z-27菌剂替代抗生素对断奶仔猪肠道消化酶、营养成分及其生长性能的影响 |
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| 引用本文： | 霍姝伊,邓祖丽颖,姜军坡,王世英,张文龙,王迈,杜国霞.Z-27菌剂替代抗生素对断奶仔猪肠道消化酶、营养成分及其生长性能的影响[J].河南农业科学,2017,46(6). |
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| 作者姓名： | 霍姝伊 邓祖丽颖 姜军坡 王世英 张文龙 王迈 杜国霞 |
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| 作者单位： | 1. 河北农业大学 生命科学学院,河北 保定,071001;2. 郑州幼儿师范高等专科学校,河南 郑州,450000;3. 石家庄市动物卫生监督所,河北 石家庄,050000 |
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| 基金项目： | 石家庄市科学技术研究与发展计划项目，河北农业大学生命科学学院大学生科技创新项目 |
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| 摘 要： | 为了评价枯草芽孢杆菌(Bacillus subtilis)Z-27菌剂替代抗生素添加剂的饲喂效果,选用96头杜洛克长白杂交断奶仔猪,随机平均分为Ⅰ组(基础日粮)、Ⅱ组(基础日粮中添加0.02‰的硫酸黏杆菌素和0.05‰的硫酸庆大霉素)、Ⅲ组(基础日粮中添加0.02‰的硫酸黏杆菌素、0.05‰的硫酸庆大霉素和0.10%的Z-27菌剂)和Ⅳ组(基础日粮中添加0.10%的Z-27菌剂),饲喂期间,每天定时观察仔猪粪便情况,计算腹泻指数。分别于正试期开始和结束时对仔猪空腹12 h称体质量,并结算剩余料,计算平均日增质量和料重比。饲喂30 d后屠宰,测定十二指肠、空肠和回肠内容物中的酸性蛋白酶、α-淀粉酶、脂肪酶及纤维素酶等消化酶活性,检测回肠、盲肠的内容物和粪便中的粗蛋白、淀粉、粗脂肪及粗纤维的含量。结果表明,与Ⅱ组相比,Ⅳ组十二指肠、空肠、回肠的酸性蛋白酶活性分别提高了74.55%(P0.05)、133.40%(P0.01)、177.61%(P0.01),α-淀粉酶活性分别提高了36.82%(P0.05)、69.72%(P0.05)、117.73%(P0.05),脂肪酶活性分别提高了30.71%(P0.05)、71.03%(P0.05)、120.38%(P0.05),纤维素酶活性分别提高了225.07%(P0.01)、244.77%(P0.01)、248.50%(P0.01);Ⅳ组仔猪粪便中的粗蛋白、淀粉、粗脂肪和粗纤维含量分别降低了29.49%(P0.05)、53.41%(P0.05)、22.71%(P0.05)和31.60%(P0.05);Ⅳ组平均日增质量提高5.35%(P0.05),料重比降低3.95%(P0.05),腹泻指数降低22.41%(P0.05)。综上,Z-27菌剂能提高仔猪肠道消化酶活性,降低仔猪肠道内容物及粪便中粗蛋白、淀粉、粗脂肪和粗纤维含量,提高饲料利用率,提高生长性能,降低仔猪腹泻指数,其效果优于抗生素添加剂。
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| 关 键 词： | 枯草芽孢杆菌 抗生素 断奶仔猪 消化酶 生长性能 腹泻指数 |
Effects of Antibiotics Substitution Agent Z-27 on Intestinal Digestive Enzymes,Nutrients and Growth Performance of Weaned Piglet |
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| HUO Shuyi,DENGZU Liying,JIANG Junpo,WANG Shiying,ZHANG Wenlong,WANG Mai,DU Guoxia.Effects of Antibiotics Substitution Agent Z-27 on Intestinal Digestive Enzymes,Nutrients and Growth Performance of Weaned Piglet[J].Journal of Henan Agricultural Sciences,2017,46(6). |
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| Authors: | HUO Shuyi DENGZU Liying JIANG Junpo WANG Shiying ZHANG Wenlong WANG Mai DU Guoxia |
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| Abstract: | In order to evaluate the feeding effect of agent Z-27 which was a substitution of antibiotics on weaned piglets,96 weaned piglets(Doric×Landrace) were randomly and averagely divided into 4 groups.Basal diet was fed to weaned piglets in the groupⅠ.Basal diet with additions of 0.02% colistin sulfate and 0.05‰ gentamicin sulfate was fed to weaned piglets in the groupⅡ.Basal diet with additions of 0.02% colistin sulfate,0.05‰ gentamicin sulfate and 0.10% agent Z-27 was fed to weaned piglets in the group Ⅲ.Basal diet with additions of 0.10% agent Z-27 was fed to weaned piglets in the group Ⅳ.Regular observation of weaned piglet feces was recorded every day during the feeding period and diarrhea index was calculated.Weights of weaned piglets and the surplus deed were obtained by fasting for 12 hours at both the beginning and the end of experimental stage and average daily gains and feed-weight ratios were calculated.After 30 days,slaughter of weaned piglets was executed.And digestive enzymes,such as acid protease,alpha-amylase,lipase and cellulose,in contents of duodenum,jejunum and ileum were determined.Then contents of nutrients,such as crude protein,starch,crude fat and crude fiber,in contents of ileum and cecum and in feces were determined.Compared with the group Ⅱ,activities of acid protease in contents of duodenum,jejunum and ileum in the group Ⅳ were increased by 74.55%(P<0.05),133.40%(P<0.01),177.61%(P<0.01),respectively.And activities of alpha-amylase in contents of duodenum,jejunum and ileum in the group Ⅳ were increased by 36.82%(P<0.05),69.72%(P<0.05),117.73%(P<0.05),respectively.Activities of lipase in contents of duodenum,jejunum and ileum in the group Ⅳ were increased by 30.71%(P<0.05),71.03%(P<0.05),120.38%(P<0.05),respectively.Activities of cellulose in contents of duodenum,jejunum and ileum in the group Ⅳ were increased by 225.07%(P<0.01),244.77%(P<0.01),248.50%(P<0.01),respectively.Contents of nutrients,such as crude protein,starch,crude fat and crude fiber in feces in the group Ⅳ were decreased by 29.49%(P<0.05),53.41%(P<0.05),22.71%(P<0.05) and 31.60%(P<0.05),respectively.The average daily gain in the group Ⅳ was increased by 5.35%(P>0.05).The feed-weight ratio and diarrhea index in the group Ⅳ were decreased by 3.95%(P>0.05) and 22.41%(P>0.05),respectively.The results showed that agent Z-27 had multifunction of improving intestinal digestive enzymes and growth performance of weaned piglets and feed utilization ratio,decreasing contents of nutrients,such as crude protein,starch,crude fat and crude fiber in intestinal tract and in feces of piglets and decreasing weaned piglet diarrhea.The effect of agent Z-27 was superior to antibiotics addition agent. |
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| Keywords: | Bacillus subtilis antibiotics weaned piglet digestive enzymes growth performance diarrhea index |
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