| 朝天椒辣椒素生物合成途径基因对茉莉酸的响应 |
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| 引用本文： | 崔莹,王宁,王鹏,李翔宇,田恬,吴辉辉,李彦磊,王晶莹,郭庆勋.朝天椒辣椒素生物合成途径基因对茉莉酸的响应[J].农业科学与技术,2017,18(1). |
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| 作者姓名： | 崔莹 王宁 王鹏 李翔宇 田恬 吴辉辉 李彦磊 王晶莹 郭庆勋 |
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| 作者单位： | 吉林大学植物科学学院,吉林长春,130062 |
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| 基金项目： | the Innovation Fund for Undergraduate of Jilin University (2016A82361).吉林大学大学生创新基金 |
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| 摘 要： | 为了探索茉莉酸(JA)对辣椒素生物合成途径基因表达的调控作用,该研究利用100μmol/L的JA处理朝天椒果实0、4、12、24和48h后,利用荧光定量PCR检测辣椒素合成途径基因mRNA表达变化,并用高效液相色谱测定处理0、1、3、6、10和15 d后果实辣椒素含量的变化.结果表明,JA可以不同程度地促进朝天椒中pal、C4h、Comt、4Cl、Hct、Pamt、Bcat、FatA和pun1基因的mRNA表达,进而促进辣椒素的合成.
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| 关 键 词： | 朝天椒 茉莉酸 辣椒素 基因表达 |
Effects of Jasmonic Acid on the Expression of Capsaicin Biosynthesis Related Genes in Chili Pepper (Capsicum annuum) |
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| Ying CUI,Ning WANG,Peng WANG,Xiangyu LI,Tian TIAN,Huihui WU,Yanlei LI,Jingying WANG,Qingxun GUO.Effects of Jasmonic Acid on the Expression of Capsaicin Biosynthesis Related Genes in Chili Pepper (Capsicum annuum)[J].Agricultural Science ＆ Technology,2017,18(1). |
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| Authors: | Ying CUI Ning WANG Peng WANG Xiangyu LI Tian TIAN Huihui WU Yanlei LI Jingying WANG Qingxun GUO |
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| Abstract: | To explore the regulatory effects of jasmonic acid (JA) on the expression of the genes involved in capsaicin biosynthesis pathway,the mRNA expression of the genes was determined by fluorescence-based quantitative PCR 0,4,12,24 and 48 h after chili peppers (Capsicum annuum var.conoides) were treated with 100 μmol/L JA,and the content of capsaicin was measured by high performance liquid chromatography (HPLC) 0,1,3,6,10 and 15 d after JA treatment.The results showed that JA upregulated the mRNA levels of pal,C4h,Comt,4Cl,Hct,Pamt,Bcat,FatA and pun1 in chili pepper,thus promoting the synthesis of capsaicin to different extents. |
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| Keywords: | Chili pepper Jasmonic acid Capsaicin Gene expression |
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