| 马拉硫磷降解菌株MN-13的降解性能测定及其培养基优化 |
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| 引用本文： | 高小朋,包本开,杨佳娟,刘蕊.马拉硫磷降解菌株MN-13的降解性能测定及其培养基优化[J].河南农业科学,2017,46(9). |
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| 作者姓名： | 高小朋 包本开 杨佳娟 刘蕊 |
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| 作者单位： | 延安大学,陕西 延安,716000 |
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| 基金项目： | 国家大学生创新创业训练计划项目，陕西省大学生创新创业训练计划项目 |
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| 摘 要： | 为了利用微生物降解土壤中残留的有机磷农药,采用磷钼蓝比色法测定了菌株MN-13降解马拉硫磷及其他有机磷农药的性能,并通过单因素选择、正交优化试验对试验菌株的生长培养基进行了优化。结果表明,试验菌株MN-13对25%马拉硫磷、40%氧化乐果、50%乙基对硫磷的降解率分别达到91.52%、69.24%、49.68%。培养基优化中,通过单因素试验选择的碳源、氮源、NaCl及pH值的中心点分别为葡萄糖1.5%、牛肉膏1.0%、NaCl 0.5%、pH值8.0;以此为基础进行正交试验,优化后培养基组成为葡萄糖1.5%、牛肉膏1.2%、NaCl 0.6%、pH值8.5,菌体OD_(600)比优化前提高了33.1%。综上,MN-13菌株可有效降解马拉硫磷等有机磷农药,其在优化后的培养基中生长量有所提高。
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| 关 键 词： | 马拉硫磷 微生物降解 MN-13菌株 降解率 培养基 优化 |
Degradation Property Characterization and Culture Medium Optimization of Malathion-Degrading Strain MN-13 |
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| GAO Xiaopeng,BAO Benkai,YANG Jiajuan,LIU Rui.Degradation Property Characterization and Culture Medium Optimization of Malathion-Degrading Strain MN-13[J].Journal of Henan Agricultural Sciences,2017,46(9). |
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| Authors: | GAO Xiaopeng BAO Benkai YANG Jiajuan LIU Rui |
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| Abstract: | In order to solve the serious non-point source soil pollution problem caused by pesticides and use microbial strains to degrade organophosphorus residues in soil,molybdenum blue spectrophotometry was used to test the performance of strain MN-13 to degrade malathion and other organophosphorus pesticides.At the same time,the methods of single factor selection and orthogonal optimization were applied to optimize the medium.The experimental results showed that the degradation rates of strain MN-13 on 25％ malathion,40％ omethoate and 50％ ethyl parathion were 91.52％,69.24％ and 49.68％ respectively.By single factor experiments,carbon source,nitrogen source,NaCl and pH of medium were preliminarily determined as glucose 1.5％,beef extract 1.0％,NaCl 0.5％ and pH 8.0.After the orthogonal experiment,the medium composition was optimized as follows:glucose 1.5％,beef extract 1.2％,NaCl 0.6％ and pH 8.5.The OD600 value of this strain was increased by 33.1％ after optimization.From the above results,it was concluded that the strain MN-13 could effectively degrade malathion and other organophosphorus pesticides,and it grew better after medium optimization. |
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| Keywords: | malathion microbial degradation MN-13 degradation rate medium optimization |
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