| 黄芪和板蓝根超微粉的粒径观察及其抗鸡新城疫病毒效果 |
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| 引用本文： | 于明鹤,史秋梅,柴铁瑛,柴楠楠,王会敏,高光平,高桂生,张艳英,高景龙.黄芪和板蓝根超微粉的粒径观察及其抗鸡新城疫病毒效果[J].河南农业科学,2017,46(5). |
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| 作者姓名： | 于明鹤 史秋梅 柴铁瑛 柴楠楠 王会敏 高光平 高桂生 张艳英 高景龙 |
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| 作者单位： | 1. 文安县畜牧兽医局,河北文安,065800;2. 河北科技师范学院河北省预防兽医学重点实验室,河北昌黎,066600;3. 承德县农牧局,河北承德,067499;4. 中红三融集团有限公司,河北承德,067499 |
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| 基金项目： | 科技部农业成果转化项目，河北省科技厅科技支撑计划项目，河北省科技厅奖励性补助项目，承德市科技局计划项目 |
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| 摘 要： | 为探讨黄芪、板蓝根超微粉的抗病毒作用,应用激光粒度分析仪和扫描电镜观察了黄芪和板蓝根超微粉的粉体粒度及其破壁情况,通过鸡胚接种试验观察2种中草药超微粉对鸡新城疫病毒的抑制作用。结果表明:黄芪和板蓝根超微粉的粒度分布中心D_(50)分别为10.10μm和8.31μm;2种中草药超微粉的破壁情况良好,电镜下只能观察到不完整的细胞壁,为组织碎片。黄芪和板蓝根超微粉对新城疫病毒最小抑制剂量分别为1 562.5、781.2μg/m L,以板蓝根抗病毒效果较好。
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| 关 键 词： | 黄芪 板蓝根 超微粉 新城疫病毒 |
Size Observation and Anti-NDV Activity of Ultrafine Particle of Radix Astragali and Radix Isatidis |
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| YU Minghe,SHI Qiumei,CHAI Tieying,CHAI Nannan,WANG Huimin,GAO Guangping,GAO Guisheng,ZHANG Yanying,GAO Jinglong.Size Observation and Anti-NDV Activity of Ultrafine Particle of Radix Astragali and Radix Isatidis[J].Journal of Henan Agricultural Sciences,2017,46(5). |
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| Authors: | YU Minghe SHI Qiumei CHAI Tieying CHAI Nannan WANG Huimin GAO Guangping GAO Guisheng ZHANG Yanying GAO Jinglong |
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| Abstract: | To investigate the antiviral effect of ultrafine powders of Radix Astragali and Radix Isatidis,the size and the broken wall of ultrafine powders of Radix Astragali and Radix Isatidis by laser particle size analyzer and scanning electron microscope were observed and the inhibitory effects on Newcastle disease virus(NDV) were observed through the inoculation of chicken embryo.The results showed that the partical size distribution center D50 of ultrafine powders of Radix Astragali and Radix Isatidis were 10.10 μm and 8.31 μm,respectively.The broken wall condition was good,and the incomplete cell wall(pieces of tissues) could only be observed under electron microscope.The minimum inhibitory concentration to NDV of Radix Astragali and Radix Isatidis were 1 562.5 μg/mL and 781.2 μg/mL respectively.The antiviral effect of Radix Isatidis was better. |
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| Keywords: | Radix Astragali Radix Isatidis ultrafine particle NDV |
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