| 酵母双杂交筛选小麦TaMBD2互作蛋白 |
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| 引用本文： | 凌娜,杨艳敏,侯江涛,孟凡荣.酵母双杂交筛选小麦TaMBD2互作蛋白[J].河南农业科学,2017,46(8). |
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| 作者姓名： | 凌娜 杨艳敏 侯江涛 孟凡荣 |
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| 作者单位： | 1. 商丘学院风景园林学院,河南商丘,476000;2. 河南农业大学生命科学学院,河南郑州,450002 |
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| 摘 要： | 为探讨小麦甲基结合域蛋白(TaMBD2)在植物生长发育过程中的调控功能,以TaMBD2基因的全长cDNA为模板,构建诱饵载体pGBKT7-TaMBD2,利用酵母双杂交系统从小麦cDNA文库中筛选TaMBD2互作蛋白。结果共筛选到91个菌斑显蓝色的克隆,对其进行菌液PCR检测并测序,然后在NCBI上进行BLAST比对分析,共获得8个可能与TaMBD2互作的蛋白,分别为二磷酸核苷激酶(NDPK)、ENTH结构域蛋白、SIAN蛋白、Agenet结构域蛋白、C2H2型锌指蛋白、磷酸激酶和2个假定蛋白,其中最有可能的TaMBD2互作蛋白为NDPK。这些候选蛋白主要参与细胞信号传导、抗逆、能量代谢和蛋白质运输等。其中,检测结果中参与植物抗逆胁迫的蛋白质为主要互作蛋白,所占比例为54.9%;参与蛋白质运输的互作蛋白所占比例为25.3%,其余互作蛋白所占比例较小。因此,推测TaMBD2可能主要参与植物对干旱、低温和高盐等非生物胁迫逆境的响应及调控。
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| 关 键 词： | 小麦 酵母双杂交 TaMBD2 互作蛋白 |
Screening of Interacted Proteins of TaMBD2 in Wheat by Yeast Two-hybrid System |
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| LING Na,YANG Yanmin,HOU Jiangtao,MENG Fanrong.Screening of Interacted Proteins of TaMBD2 in Wheat by Yeast Two-hybrid System[J].Journal of Henan Agricultural Sciences,2017,46(8). |
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| Authors: | LING Na YANG Yanmin HOU Jiangtao MENG Fanrong |
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| Abstract: | In order to understand more regulatory functions of methyl-binding domain protein TaMBD2 during the growth and development in wheat,bait vector pGBKT7-TaMBD2 was constructed with the full length cDNA of TaMBD2 gene as template,and the interacted proteins of TaMBD2 from wheat cDNA library were screened by yeast two-hybrid system.The result showed that a total of 91 blue clones were screened,which were analyzed through PCR and homology analysis using the BLAST in NCBI,and eight possible interacted proteins of TaMBD2 were obtained,which were nucleoside diphosphate kinase (NDPK),epsin N-terminal homology domain-containing protein,seven in absentia family protein,Agenet domain-containing protein,zinc finger C2H2 type family protein,phosphate kinase and two hypothetical proteins.The most likely interacted protein of TaMBD2 was NDPK.These candidate proteins were related to mediate cell signal transduction,stress resistance,energy metabolism and protein transport,etc.Among them,the proteins involved in resistance to plant stress were the main interacted protein,the proportion was 54.9％;the proportion of proteins participated in protein transport was 25.3％,and the proportion of the rest interacted proteins was smaller.Therefore,TaMBD2 may mainly participate in response and regulation to abiotic stress,such as drought,low temperature and high salt. |
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| Keywords: | wheat yeast two-hybrid TaMBD2 interacted proteins |
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