| 猪流行性腹泻病毒RT-PCR检测方法的建立与应用 |
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| 引用本文： | 徐引弟,鲁智远,王治方,李海利,张青娴,朱文豪,焦文强,郎利敏,王克领.猪流行性腹泻病毒RT-PCR检测方法的建立与应用[J].河南农业科学,2017,46(9). |
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| 作者姓名： | 徐引弟 鲁智远 王治方 李海利 张青娴 朱文豪 焦文强 郎利敏 王克领 |
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| 作者单位： | 河南省农业科学院 畜牧兽医研究所,河南 郑州,450002 |
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| 基金项目： | 河南省科技攻关计划项目，河南省农业科学院自主创新基金项目 |
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| 摘 要： | 为了建立猪流行性腹泻病毒(PEDV)快速、特异的检测方法,减少猪流行性腹泻(PED)给养猪业带来的损失,根据PEDV的S基因序列设计1对引物,优化扩增条件,建立了针对PEDV的RTPCR检测方法。结果显示,建立的检测方法可特异地检测出PEDV。应用建立的检测方法对2016年河南地区38家发生腹泻的猪场的256份病料进行检测,结果表明,阳性猪场为30家,阳性样本为157份,检出率高于试纸条检测方法。可见,建立的检测方法特异性强,可用于PEDV感染疑似病例的诊断及流行病学调查。
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| 关 键 词： | 猪流行性腹泻 RT-PCR 检测 |
Development of RT-PCR to Detect Porcine Epidemic Diarrhea |
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| XU Yindi,LU Zhiyuan,WANG Zhifang,LI Haili,ZHANG Qingxian,ZHU Wenhao,JIAO Wenqiang,LANG Limin,WANG Keling.Development of RT-PCR to Detect Porcine Epidemic Diarrhea[J].Journal of Henan Agricultural Sciences,2017,46(9). |
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| Authors: | XU Yindi LU Zhiyuan WANG Zhifang LI Haili ZHANG Qingxian ZHU Wenhao JIAO Wenqiang LANG Limin WANG Keling |
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| Abstract: | In order to develop a rapid and specific test method for porcine epidemic diarrhea,reduing the losses to the pig industry caused by PEDV,a pair of primers were designed accroding to PEDV S gene sequence.Amplification conditions were optimized,RT-PCR method to detect PEDV was established.The method could specifically detect the RNA of PEDV.The method was used to test 256 samples of 38 Henan farms in 2016.The results showed that 157 samples were positive,30 farms were postivie,and the detection rate was higher than that of strip method.It indicated that the specificity of RT-PCR method to detect PEDV was good and could be used to diagnosis of suspected cases of infection and epidemiological investigation of PEDV. |
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| Keywords: | PED RT-PCR detection |
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