| 重组热带螨Blo t 21变应原诱导小鼠变态反应气道炎症模型的建立 |
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| 引用本文： | 肖正泮,刘金莹,韦双双,王大勇,裴业春.重组热带螨Blo t 21变应原诱导小鼠变态反应气道炎症模型的建立[J].热带生物学报,2017,8(3). |
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| 作者姓名： | 肖正泮 刘金莹 韦双双 王大勇 裴业春 |
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| 作者单位： | 1. 海南大学热带农林学院动物科技学院/海南省热带动物繁育与疫病研究重点实验室,海口,570228;2. 海南大学热带农林学院生物学院,海口,570228 |
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| 基金项目： | 国家自然科学基金青年项目，海南省科技项目资助 |
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| 摘 要： | 为了建立热带螨重组变应原Blo t 21(rBlo t 21)诱导小鼠变态反应气道炎症模型,笔者利用从NCBI Gen Bank库里获得的热带螨Blo t 21基因序列,进行了序列优化与全基因合成,构建了原核表达载体PQE80LrBlo t 21,在大肠杆菌内诱导表达rBlo t 21,利用镍柱亲和层析纯化rBlo t 21蛋白。12只BALB/c小鼠随机分为对照组(PBS组)与诱导组(rBlo t 21组),每组6只,通过腹腔注射致敏与气道诱导后,分别通过酶联免疫吸附试验(ELISA)检测血清总IgE水平;鼠尾静脉注射变应原诱发应激性过敏反应;HE染色观察小鼠肺部炎症严重程度。本试验成功表达与纯化了重组热带螨变应原Blo t 21。利用纯化的rBlo t 21诱导小鼠变态反应气道炎症模型,与PBS组相比,rBlo t 21组的小鼠血清总IgE水平显著升高,肺部组织细胞侵润明显增加(P0.05)。原核表达纯化的重组Blo t 21变应原能够诱导小鼠产生变态反应气道炎症。
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| 关 键 词： | 热带螨 重组Blo t 21蛋白 气道炎症 |
The Development of Airway Inflammation Murine Model Induced by Recombinant Blomia tropicalis Group 21 Allergen |
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| XIAO Zhengpan,LIU Jinying,WEI Shuangshuang,WANG Dayong,PEI Yechun.The Development of Airway Inflammation Murine Model Induced by Recombinant Blomia tropicalis Group 21 Allergen[J].Journal of Tropical Biology,2017,8(3). |
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| Authors: | XIAO Zhengpan LIU Jinying WEI Shuangshuang WANG Dayong PEI Yechun |
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| Abstract: | An attempt was made to develop airway inflammation murine model induced by the recombinant Blot 21 allergen.The Blot 21 gene sequences were obtained from NCBI GenBank,then optimized,synthesized and inserted into the prokaryotic expression vector PQE80L.The recombinant Blo t 21 was expressed in E.coli bacteria,and purified by NI-NTA agarose.Twelve BALB/c mice were divided randomly into control group (PBS group,n =6) and rBlo t 21-induced group (rBlo t 21 group,n =6).After sensitization by intraperitoneal injection (i.p) and challenged by intratracheal instillation of rBlo t 21,the total serum IgE levels were measured by ELISA;the lungs were fixed and stained with haematoxylin and eosin (H&E).The recombinant Blot 21 allergen was expressed successfully in E.coli and purified by Ni-NTA affinity chromatography.Furthermore,the airway inflammation murine model were induced by the recombinant Blot 21 allergen.Compared with the control group (PBS group),the total serum IgE levels rose obviously in the rBlo t 21 group (p ＜0.005).Mice in the rBlo t 21 group showed inflammatory cell infiltration in airway by haematoxylin and eosin (H&E).The airway inflammation murine model was induced successfully by the recombinant Blot 21 allergen. |
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| Keywords: | Blomia tropicalis recombinant Blo t 21 airway inflammation |
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