| 兔巴氏杆菌与大肠杆菌混合感染病例诊断及药敏试验 |
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| 引用本文： | 朱芝秀,蒋新华,邓舜洲,林敏,刘小兰,柳仲,彦超.兔巴氏杆菌与大肠杆菌混合感染病例诊断及药敏试验[J].农业科学与技术,2017,18(2). |
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| 作者姓名： | 朱芝秀 蒋新华 邓舜洲 林敏 刘小兰 柳仲 彦超 |
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| 作者单位： | 1. 江西农业大学动物科技学院,江西南昌,3300452;2. 南昌金牧生物有限公司,江西南昌,330013 |
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| 基金项目： | 江西农业大学横向课题，江西省教育厅(GJJ150416)课题，江西省科技厅(20122BBF60082)课题.Supported by Crosswise Project of Jiangxi Agricultural University，Project of Education Department of Jiangxi Province，Project of Science and Technology Department of Jiangxi Province |
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| 摘 要： | 为了诊断患病死亡兔,对死亡兔进行临床解剖,细菌分离培养,获得DX01、DX02、JX01、JX02等4株菌.按照常规生化鉴定法,对分离菌进行生理生化实验,结果:DX01、DX02对甘露醇、乳糖、葡萄糖、麦芽糖均产酸产气,不发酵蔗糖,枸橼酸钠、尿素、V-P、硫化氢、明胶等反应均为阴性,靛基质、M-R、硝酸盐等反应均为阳性,与大肠杆菌标准株完全一致;JX01、JX02均发酵甘露醇、蔗糖、葡萄糖、麦芽糖,不发酵乳糖,靛基质、M-R、V-P、硫化氢、明胶等反应均为阴性,硝酸盐反应为阳性,与巴氏杆菌标准株基本一致,JX01、JX02进一步经过16SrDNA检测与测序分析,与巴氏杆菌同源性为100％.分离菌动物接种试验结果,试验组小白鼠在2日内均死亡.死亡小白鼠体内均分离出了原培养物.结论:DX01、DX02为大肠杆菌,JX01、JX02为大肠杆菌,患病死亡兔由大肠杆菌和巴氏杆菌混合感染引起的.按照琼脂扩散法做药敏实验,结果DX01、DX02只对卡那霉素、氧氟沙星、甲氧嘧啶敏感,对其他的药物不敏感;JX01、JX02对氧氟沙星、甲氧嘧啶、庆大霉素、呋喃唑酮、强力霉素、链霉素、复方新诺明、大观霉素等敏感,对头孢拉啶等中度敏感.治疗该病临床用药首选氧氟沙星、甲氧嘧啶.
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| 关 键 词： | 兔 大肠杆菌 巴氏杆菌 诊断 |
Diagnose of Rabbit Pasteurellosis and Colibacillosis Mixed Infection and Drug Sensitive Test |
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| Zhixiu ZHU,Xinhua JIANG,Shunzhou DENG,Min LIN,Xiaolan LIU,Zhong LIU,Chao YAN.Diagnose of Rabbit Pasteurellosis and Colibacillosis Mixed Infection and Drug Sensitive Test[J].Agricultural Science ＆ Technology,2017,18(2). |
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| Authors: | Zhixiu ZHU Xinhua JIANG Shunzhou DENG Min LIN Xiaolan LIU Zhong LIU Chao YAN |
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| Abstract: | In order to diagnose diseased rabbits,dead rabbits were dissected,and 4 strains,DX01,DX02,JX01 and JX02 were obtained from bacterial isolation and culture.According to conventional biochemical method,physiological-biochemical test showed that DX01 and DX02 could produce acids and gases in the presence of mannitol,lactose,glucose and maltose,and did not ferment sucrose,reactions of sodium citrate,urea,V-P,hydrogen sulfide and gelatin were negative,while reactions of indole,M-R and nitrate were positive,which were in accordance with the characteristics of colibacillus;and JX01 and JX02 both fermented mannitol,sucrose,glucose,maltose,and did not ferment lactose,reactions of indole,M-R,V-P,hydrogen sulfide and gelatin were negative,while the reaction of nitrate was positive,which were in accordance with the characteristics of Pasteurella.16S rDNA detection and sequencing showed that JX01 and JX02 shared 100％ homology with Pasteurella.The animal inoculation test of isolated bacteria showed that the mice in the experiment groups all died within 2 d.Original cultures were isolated from dead mice.DX01 and DX02 were identified as colibacillus,JX01 and JX02 were identified as Pasteurella,and the death of diseased rabbits was caused by mixed infection of pasteurellosis and colibacillosis.Drug sensitive test showed that DX01 and DX02 were only sensitive to kanamycin,ofloxacin and sulfamethoxydiazine,and not sensitive to other drugs,and JX01 and JX02 were sensitive to ofloxacin,sulfamethoxydiazine,gentamicin,furazolidone,doxycycline,streptomycin,trimoxazole and spectinomycin,and were moderately sensitive to cefradine.Ofloxacin and sulfamethoxydiazine are preferable for the clinical treatment of this disease. |
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| Keywords: | Rabbit Colibacillus Pasteurella Diagnosis |
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