| 木薯花粉原位萌发及柱头可授性观察 |
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| 引用本文： | 周亚秋,孙琪,陈霞,赖杭桂,陈松笔,叶剑秋.木薯花粉原位萌发及柱头可授性观察[J].热带生物学报,2017,8(1). |
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| 作者姓名： | 周亚秋 孙琪 陈霞 赖杭桂 陈松笔 叶剑秋 |
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| 作者单位： | 1. 海南大学农学院,海口,570228;2. 中国热带农业科学院品种资源研究所,海南儋州,571737 |
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| 基金项目： | 国家自然科学基金项目，海南大学中西部计划学科重点领域建设项目，海南大学科研启动基金项目 |
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| 摘 要： | 以木薯华南5号(SC5)和华南7号(SC7)品种为实验材料,应用花粉活体原位萌发技术及联苯胺-过氧化氢法雌蕊柱头可授性检测技术,对木薯花粉萌发及花粉管发育特征进行观察,并分析木薯品种内自交及品种间异交花粉管萌发过程的差异,以确定木薯雌蕊柱头的最佳授粉时期。结果表明,木薯在同品种自交和异品种杂交上均存在授粉亲和性上的差异,异交授粉无论在花粉萌发速度及萌发率上均具有明显的优势。雌蕊柱头的最佳授粉时间为雌花开放后2~5 h,5~24 h内柱头具较强可授性,时间越长可授性越差,72 h后柱头不再具有可授性。
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| 关 键 词： | 木薯 花粉 原位萌发 柱头 可授性 |
Pollen Germination in Situ and Stigma Receptivity of Cassava |
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| ZHOU Yaqiu,SUN Qi,CHEN Xia,LAI Hanggui,CHEN Songbi,YE Jianqiu.Pollen Germination in Situ and Stigma Receptivity of Cassava[J].Journal of Tropical Biology,2017,8(1). |
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| Authors: | ZHOU Yaqiu SUN Qi CHEN Xia LAI Hanggui CHEN Songbi YE Jianqiu |
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| Abstract: | The pollen germination and stigma receptivity is an important part of plant reproduction,and it is the basis for the artificial pollination in reproduction and crossbreeding.The pollen germination rate and pollen tube growth of cassava varieties SC5 and SC7 were observed by using the method for pollen germination in situ and in vivo,and the stigma receptivity was detected by using the benzidine-hydrogen peroxide test.The differences of the pollen tube germination process in selfng or crossing of these two cassava varieties were analyzed to determine the best pollination time for the stigmas of the pistil of cassava.The results show that there are differences in pollination affinity of the cassava varieties in the self-and cross-pollination,and that cross pollination had obvious advantages in pollen germination speed and rate.The best pollination time for the stigma was 2-5 h after the female flowers opened,and the stigma receptivity was the highest within 5-24 h.The stigma receptivity turned to be weaker with time and was no longer available after 72 h. |
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| Keywords: | Cassava pollen germination in situ stigma receptivity |
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