| 应用正交试验优化猪细小病毒N株在IBRS-2细胞转瓶培养 |
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| 引用本文： | 卢冰霞,何颖,赵武,梁家幸,段群棚,蒋冬福,卢敬专,闭炳芬,周英宁,秦毅斌,李斌,苏乾莲,陈忠伟.应用正交试验优化猪细小病毒N株在IBRS-2细胞转瓶培养[J].农业科学与技术,2017,18(5). |
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| 作者姓名： | 卢冰霞 何颖 赵武 梁家幸 段群棚 蒋冬福 卢敬专 闭炳芬 周英宁 秦毅斌 李斌 苏乾莲 陈忠伟 |
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| 作者单位： | 广西壮族自治区兽医研究所/广西兽医生物技术重点实验室,广西南宁,530001 |
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| 基金项目： | 广西公益性科研院所基本科研业务费专项项目，广西水产畜牧科技项目(桂渔牧科201528030 |
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| 摘 要： | 利用正交试验对猪细小病毒(PPV)N株在IBRS-2细胞转瓶培养中的培养条件进行优化,为PPV N株弱毒疫苗的大量培养工艺提供参考.设细胞培养液pH值(7.0、7.2和7.4)、接毒时间(0、24和48 h)、接毒剂量(0.10％、1.00％和10.00％)、收毒时间(48、72和96 h)等因子和水平.结果表明,PPV N株转瓶培养的最佳培养条件组合为细胞培养液pH值7.2,采用同步接毒,接毒剂量为0.10％,收毒时间为接毒后96 h.其中收毒时间是影响PPV N株毒价的最主要因素.
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| 关 键 词： | 猪细小病毒 转瓶培养 优化 正交试验 |
Optimization of IBRS-2 Cells Culturing Conditions for Cultivation of Porcine Parvovirus N Strain Using Roller Bottle |
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| Bingxia LU,Ying HE,Wu ZHAO,Jiaxing LIANG,Qunpeng DUAN,Dongfu JIANG,Jingzhuan LU,Bingfen BI,Yingning ZHOU,Yibin QIN,Bin LI,Qianlian SU,Zhongwei CHEN.Optimization of IBRS-2 Cells Culturing Conditions for Cultivation of Porcine Parvovirus N Strain Using Roller Bottle[J].Agricultural Science ＆ Technology,2017,18(5). |
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| Authors: | Bingxia LU Ying HE Wu ZHAO Jiaxing LIANG Qunpeng DUAN Dongfu JIANG Jingzhuan LU Bingfen BI Yingning ZHOU Yibin QIN Bin LI Qianlian SU Zhongwei CHEN |
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| Abstract: | The aim was to optimize the rolier bottle culture system of IBRS-2 cells for cultivation of porcine parvovirus (PPV) N strain using a four-factor three-level or-thogonal test (cell culture medium pH value:7.0,7.2 and 7.4;inoculation time:0,24 and 48 h;inoculation dose:0.10％,1.00％ and 10.00％;harvest time:48,72 and 96 h).The results showed that the optimal conditions were as follows:cell culture medium pH value of 7.2,synchronous inoculation,inoculation dose of 0.10％ and culture time of 96 h.Among the four factors,culture time was the most important factor affecting the virulence titer of PPV N strain. |
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| Keywords: | Porcine parvovirus (PPV) Roller bottle culture Optimization Orthogonal test |
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