| 正交试验优化重结晶法纯化芝麻素的工艺及HPLC成分分析 |
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| 引用本文： | 刘末,周仁杰,吴静,雷红.正交试验优化重结晶法纯化芝麻素的工艺及HPLC成分分析[J].农业科学与技术,2017,18(7). |
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| 作者姓名： | 刘末 周仁杰 吴静 雷红 |
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| 作者单位： | 1. 南京财经大学食品科学与工程学院,江苏南京,210023;2. 合肥工业大学食品科学与工程学院,安徽合肥,230009;3. 南京财经大学食品科学与工程学院,江苏南京210023;合肥工业大学食品科学与工程学院,安徽合肥230009 |
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| 基金项目： | Natural Science Foundation of Anhui Province (1608085MC53).安徽省自然科学基金 |
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| 摘 要： | 目的]采用重结晶法纯化高纯度的芝麻素,得到最优的重结晶纯化条件,并分析其含量纯度.方法]以芝麻粕为实验材料,醇提、大孔树脂吸附、乙醇结晶、丙酮-异丙醇重结晶方法纯化芝麻素.HPLC法测定芝麻素含量.应用重结晶纯化技术,采用单因素和正交试验优化方法,选取芝麻素初结晶料液比、热溶解温度、下降温度三个单因素作为考察指标,优化芝麻素重结晶条件.结果]通过单因素和正交试验结果分析得到,最优芝麻素重结晶条件为:料液比1:10,热溶解温度45℃,结晶温度25 ℃,在此条件下重结晶样品经HPLC分析芝麻素含量达到95.26％.结论]采用重结晶法纯化技术,通过单因素和正交试验法优化实验,并经HPLC含量分析,得到了高纯度的芝麻素结晶.
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| 关 键 词： | 芝麻素(SES) 重结晶法 正交试验法 HPLC |
Optimal Conditions for the Purification of Sesamin by Recrystallization |
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| Mo LIU,Renjie ZHOU,Jing WU,Hong LEI.Optimal Conditions for the Purification of Sesamin by Recrystallization[J].Agricultural Science ＆ Technology,2017,18(7). |
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| Authors: | Mo LIU Renjie ZHOU Jing WU Hong LEI |
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| Abstract: | Obiective] Sesamin has been widely used in healthy food and medicines in recent years due to its prominent biological functions.This study was aimed to determine the optimal parameters for sesamin recrystallization,to obtain high-quality and high-purity sesamin.Method] The effects of solid-liquid ratio,heating temperature and cooling temperature on sesamin recrystallization were investigated by single-factor experiments and orthogonal array testing (ORT).The purity of resulting sesamin was investigated by HPLC.Result] The optimal conditions for the purification of sesamin were solid-liquid ratio of 1:10,heating temperature of 45 ℃ and cooling temperature of 25 ℃.Under these conditions,the purity of sesamin reached 95.26％.Conclusion] Recrystallization is an effective technique to purify sesamin from sesame meal.Moreover,HPLC is also an ideal method for analyzing the purity of sesamin. |
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| Keywords: | Sesamin Recrystallization Orthogonal array testing HPLC |
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