| 奶牛新孢子虫病的巢式PCR诊断及ELISA检测 |
| |
| 引用本文： | 段保擎,王天奇,钱伟锋,闫文朝,彭曦冉.奶牛新孢子虫病的巢式PCR诊断及ELISA检测[J].河南农业科学,2017,46(2). |
| |
| 作者姓名： | 段保擎 王天奇 钱伟锋 闫文朝 彭曦冉 |
| |
| 作者单位： | 河南科技大学 动物科技学院,河南 洛阳,471003 |
| |
| 基金项目： | 河南科技大学博士科研启动基金项目 |
| |
| 摘 要： | 为确定焦作某奶牛场奶牛流产是否与新孢子虫感染有关,对采自该奶牛场流产奶牛的胎牛组织进行新孢子虫Nc5基因巢式PCR检测诊断,并用ELISA方法对该奶牛场不同年龄阶段奶牛血清样品进行新孢子虫抗体检测。结果显示,在4例流产胎牛样本中有3例检出新孢子虫DNA;新孢子虫血清抗体阳性率达44.80%(112/250),其中,经产奶牛阳性率为55.40%(77/139),未经产奶牛或犊牛阳性率为31.53%(35/111);经产奶牛中有流产史的阳性率为73.17%(30/41),其余未发生过流产的阳性率为47.96%(47/98)。综上推断,新孢子虫是导致该奶牛场奶牛流产的主要病原。
|
| 关 键 词： | 奶牛 新孢子虫 巢式PCR 流产 抗体 |
Diagnosis of Neospora caninum Infection in Cattle Using Nested PCR and Its Detection by ELISA |
| |
| DUAN Baoqing,WANG Tianqi,QIAN Weifeng,YAN Wenchao,PENG Xiran.Diagnosis of Neospora caninum Infection in Cattle Using Nested PCR and Its Detection by ELISA[J].Journal of Henan Agricultural Sciences,2017,46(2). |
| |
| Authors: | DUAN Baoqing WANG Tianqi QIAN Weifeng YAN Wenchao PENG Xiran |
| |
| Abstract: | In order to determine the prevalence of Neospora canium in cattle from a dairy farm in Jiaozuo, aborted fetuses samples were tested by nested PCR,and serum samples of cattle with different ages were tested for antibodies to N. caninum by using an enzyme-linked immunosorbent assay( ELISA) . The results showed that N. caninum DNA was detected in three of the four foetuses. N. caninum seroprevalence was 44. 80%(112/250) in dairy cows. The seroprevalence was 55. 40%(77/139) for dairy cows with preg-nancy,31. 53%(35/111) for dairy cows with no pregnancy or calf. The seroprevalence of dairy cows with an aborting history was 73. 17%(30/41),47. 96%(47/98) for dairy cows without abortion. Combined with the clinical manifestations of aborting dams and management in this dairy farm, N. caninum was thought to be the most potential cause of abortions. |
| |
| Keywords: | dairy cow Neospora canium nested PCR abortion antibody |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
