| 姜黄素对菊花DNA甲基化及其生长发育的影响 |
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| 引用本文： | 刘艳华,李忠爱,李杰,刘晓,王子成.姜黄素对菊花DNA甲基化及其生长发育的影响[J].河南农业科学,2017,46(5). |
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| 作者姓名： | 刘艳华 李忠爱 李杰 刘晓 王子成 |
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| 作者单位： | 1. 河南大学生命科学学院,河南开封,475004;2. 河南省邓州市刘集镇农业服务中心,河南邓州,474150 |
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| 摘 要： | 采用0、100、200、400μmol/L不同浓度的姜黄素对菊花神马组培苗进行处理,利用甲基化敏感扩增多态性技术(MSAP)分析DNA甲基化水平,并观察植株生长状况。结果表明:姜黄素处理对菊花花期的影响达到了显著水平,以200μmol/L姜黄素处理的菊花最早开花,花期可提前5~8 d。MSAP分析结果表明,经姜黄素处理的菊花组培苗的DNA甲基化比率随姜黄素浓度的升高而降低,在0、100、200、400μmol/L姜黄素处理下,甲基化比率分别为58.40%、50.44%、48.24%和46.00%。经姜黄素处理的菊花组培苗矮化萎蔫,叶片数减少,分芽能力减弱。移植至室外后,植株矮化等表型抑制作用稳定遗传。表明姜黄素处理菊花可使其基因组甲基化水平降低,产生表观遗传变异,导致提前开花。
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| 关 键 词： | 菊花 姜黄素 DNA甲基化 变异 |
Effects of Curcumin on DNA Methylation and Development of Chrysanthemum |
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| LIU Yanhua,LI Zhongai,LI Jie,LIU Xiao,WANG Zicheng.Effects of Curcumin on DNA Methylation and Development of Chrysanthemum[J].Journal of Henan Agricultural Sciences,2017,46(5). |
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| Authors: | LIU Yanhua LI Zhongai LI Jie LIU Xiao WANG Zicheng |
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| Abstract: | In this study,the chrysanthemum plantlets were treated with 0,100,200,400 μmol/L of curcumin,methylation sensitive amplified polymorphism(MSAP) technology was used to analyse the DNA methylation level,and the plant growth status was observed.The results showed that the effect of curcumin on the flowering time of chrysanthemum reached a significant level,the earliest flowering time with 200 μmol/L was 5-8 days earlier than the control.MSAP results showed that the DNA methylation rate of treated tissue culture seedlings decreased with the increase of curcumin concentration,and the methylation rates were 58.40％,50.44％,48.24％ and 46.00％ under the curcumin concentration of 0,100,200,400 μmol/L,respectively.Seedlings plants showed dwarf,easily wilt,with less leaves and more weak buds.After transplantation to the outside,dwarfing phenotype was inherited stably.In conclusion,curcumin treatment can reduce genomic methylation level and produce epigenetic variation,leading to early flowering of chrysanthemum. |
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| Keywords: | chrysanthemum curcumin DNA methylation variation |
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