| 花骨鱼气单胞菌分离鉴定及体外抑菌试验(英文) |
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| 引用本文： | 朱芝秀,徐添乐,邓舜洲,陈君,陈蕊,谢莉敏,黄志琴,伍思华,吴允正.花骨鱼气单胞菌分离鉴定及体外抑菌试验(英文)[J].农业科学与技术,2017,18(1). |
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| 作者姓名： | 朱芝秀 徐添乐 邓舜洲 陈君 陈蕊 谢莉敏 黄志琴 伍思华 吴允正 |
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| 作者单位： | 江西农业大学动物科技学院,江西南昌,330045 |
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| 基金项目： | the Cooperation Project，the Project of the Ministry of Education of Jiangxi Province，the Project of the Ministry of Science and Technology of Jiangxi Province (20122BBF60082).合作课题，江西省教育厅(GJJ150416)课题，江西省科技厅(20122BBF60082)课题 |
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| 摘 要： | 目的]为了快速找到高免药物,阻止野生花骨鱼的不断死亡。方法]用常规生化法和分子生物鉴定法将病死花骨鱼进行细菌分离鉴定,用琼脂扩散法做药敏实验和体外抑菌实验,用平板法做毒素检测,用腹腔注射法做动物实验。结果]得到12株分离菌,分离菌一致能分解葡萄糖、麦芽糖等,不能分解乳糖;能还原硝酸盐;硫化氢、靛基质等阴性;分离菌在685 bp处均有一清晰条带;分离菌皆产生了溶血素;部分菌株产生了蛋白酶;分离菌对青霉素G、阿莫西林等耐药;对庆大霉素、环丙沙星等敏感;解淀粉芽孢杆菌对分离菌均有抑制作用;实验动物12 h内皆死亡。结论]12株分离菌分别为气单胞菌属的维氏温和气单胞菌、豚鼠气单胞菌和嗜水气单胞菌;花骨鱼死亡由维氏温和气单胞菌、豚鼠气单胞菌和嗜水气单胞菌混合感染所致;治疗首选药物为环丙沙星;生态防治药物可用解淀粉芽孢杆菌。
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| 关 键 词： | 气单胞菌 分离鉴定 抑菌实验 |
Isolation,Identification and Antimicrobial Test in vitro of Aeromonas from Hemibarbus maculatus Bleeker |
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| Zhixiu ZHU,Tianle XU,Shunzhou DENG,Jun CHEN,Rui CHEN,Limin XIE,Zhiqin HUANG,Sihua WU,Yunzheng WU.Isolation,Identification and Antimicrobial Test in vitro of Aeromonas from Hemibarbus maculatus Bleeker[J].Agricultural Science ＆ Technology,2017,18(1). |
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| Authors: | Zhixiu ZHU Tianle XU Shunzhou DENG Jun CHEN Rui CHEN Limin XIE Zhiqin HUANG Sihua WU Yunzheng WU |
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| Abstract: | Objective] The aim was to quickly find high free drugs to prevent Hemibarbus maculates Bleeker from dying.Method] The conventional biochemical methods and molecular biological identification method was used to do the isolation and identification of bacteria from H.maculates,and agar diffusion method was used for the susceptibility test and in vitro bacteriostasis experiment.The toxins were detected using the plate method,and the intraperitoneal injection method was used to do animal experiments.Result] Twelve strains of isolated bacteria were obtained,which could uniformly decompose glucose,maltose,but were unable to break down lactose,and they could reduce the nitrate,and were negative in hydrogen sulfide and indole.A clear band was found in 685 bp of the isolated bacteria.And the isolated bacterial all generated hemolysins.Some strains produced protease.The isolated bacterial were resistant to penicillin G,amoxicillin,but sensitive to gentamicin,ciprofloxacin,and could be inhibited by the Bacillus amyloliquefaciens.All experimental animals died within 12 hours.Conclusion] The 12 isolates were Aeromonas veronii,Aeromonas caviae and Aeromonas hydrophila,respectively,and the deaths of H.maculates were caused by the mixed infection of Aeromonas veronii,Aeromonas caviae and Aeromonas hydrophila.The first choice drug for the treatment was ciprofloxacin,and Bacillus amyloliquefaciens could be used as the drug for ecological prevention and control. |
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| Keywords: | Aeromonas Isolation and identification Antibacterial activity |
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