| 结核杆菌实时荧光定量PCR快速检测方法的建立及应用 |
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| 引用本文： | 孟茹,陈创夫,张辉,乔军,王正荣.结核杆菌实时荧光定量PCR快速检测方法的建立及应用[J].中国农学通报,2010,26(10):1-4. |
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| 作者姓名： | 孟茹 陈创夫 张辉 乔军 王正荣 |
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| 作者单位： | 石河子大学动物科技学院,新疆,石河子,832003 |
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| 摘 要： | 针对结核杆菌CFP10基因序列设计并合成了1对引物，以构建的含有该引物扩增序列的重组质粒作为阳性标准品，建立了检测CFP10基因的SYBR GreenⅠreal-time PCR方法。检测结果显示，该方法线性关系好，标准曲线的相关系数达到0.997，对初始模板的检出下限为1 ×101copies/μL，比常规PCR方法高100倍。表明建立的real-time PCR检测方法灵敏度高、特异性强、重复性好，可以用于结核杆菌CFP10的病原检测及定量分析。
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| 关 键 词： | 克伦特罗 克伦特罗 心肌 ATP 抗氧化能力 |
| 收稿时间： | 2009-12-23 |
| 修稿时间： | 2010-02-22 |
Construction of Real-time PCR quantitative rapid detection of Mycobacterium tuberculosis |
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| Meng Ru,Chen Chuangfu,Zhang Hui,Qiao Jun,Wang Zhengrong.Construction of Real-time PCR quantitative rapid detection of Mycobacterium tuberculosis[J].Chinese Agricultural Science Bulletin,2010,26(10):1-4. |
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| Authors: | Meng Ru Chen Chuangfu Zhang Hui Qiao Jun Wang Zhengrong |
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| Institution: | Shihezi University Institute of Animal Science and Technology, Shihezi Xinjiang 832003 |
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| Abstract: | According to the sequence of the Mycobacterium tuberculosis CFP10 gene to established a SYBR GreenIreal-time PCR method that could detect Mycobacterium tuberculosis CFP10 gene. Designed and synthesized a pair of CFP10 gene primer to build positive standard sample that containing the sequence of the recombinant plasmid. Test results showed that a good linear relationship of this method, correlation coefficient of this method reached to 0.997, the lowest detection threshold of this method was ×10^1copies/μL that higherl00 times than the conventional PCR method. The method has high sensitivity and strong specific and good repeatability that can be used to detect the pathogen of Mycobacterium tuberculosis and quantitative analysis. |
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| Keywords: | CFP10 |
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