| 长吻遗传多样性的RAPD分析 |
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| 引用本文： | 莫艳秀,王晓清,莫永亮.长吻遗传多样性的RAPD分析[J].南方水产,2010,6(6):77-80. |
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| 作者姓名： | 莫艳秀 王晓清 莫永亮 |
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| 作者单位： | [1]湘南学院组织胚胎学教研室,湖南郴州423043 [2]湖南农业大学动物科学技术学院,湖南长沙410128 [3]北大附中广州实验学校,广东广州510250 |
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| 摘 要： | 以洞庭湖和原种场长吻（Leiocassis longirostris）为研究对象,采用20个随机引物对10个野生个体进行了随机扩增多态性DNA（random amplified polymorphic DNA,RAPD）群体遗传多样性分析。共检测到103条带,每个引物产生的条带数在2～9之间,片段大小在0.2～3.0kb之间,多态座位比例为41.75%,2群体内个体间遗传相似系数和遗传距离分别是,洞庭湖个体间遗传相似性系数（S）0.8795～0.9833,遗传距离（D）0.0167～0.1205;原种场个体间S为0.8794～0.9892,D为0.0319～0.1108;比较2群体S为0.7983～0.9994,D为0.0167～0.3017;Nei遗传多样性指数（He）0.3269。结果表明,长吻群体内遗传多样性较为丰富。
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| 关 键 词： | 长吻 遗传多样性 随机扩增多态DNA |
RAPD analysis of genetic diversity of Leiocassis longirostris |
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| MO Yanxiu,WANG Xiaoqing,MO Yongliang.RAPD analysis of genetic diversity of Leiocassis longirostris[J].South China Fisheries Science,2010,6(6):77-80. |
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| Authors: | MO Yanxiu WANG Xiaoqing MO Yongliang |
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| Institution: | 1. Department of Histology and Embryology,Xiangnan University,Chenzhou 423043,China;2. College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;3. Affiliated High School of Peking University Guangzhou Experimental School,Guangzhou 510250,China) |
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| Abstract: | Taking wild Leiocassis longirostris from Dongting Lake and original breeding farm as samples and with 20 random primers,we conducted a RAPD analysis of genetic diversity of 10 wild individuals,finding 103 bands whose fragment size are 0.2～3.0 kb,and that each primer generates 2～9 bands with a polymorphic loci proportion of 41.75%.Genetic similarity coefficient and genetic distance within 2 populations are as follows：genetic similarity index（S） of Dongting Lake individuals varies from 0.879 5 to 0.983 3 and that of original breeding farm ones varies from 0.879 4 to 0.989 2;genetic distance（D） varies from 0.016 7 to 0.120 5 and that of original breeding farm ones varies from 0.031 9 to 0.110 8;the genetic similarity coefficient of 2 populations is 0.798 3～0.999 4 and the genetic distance is 0.016 7～0.301 7;Nei genetic diversity index is 0.326 9.The results indicate that L.longirostris has relatively high genetic diversity within populations. |
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| Keywords: | Leiocassis longirostris genetic diversity RAPD |
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