| 白腐菌产漆酶培养条件的响应面法优化 |
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| 引用本文： | 毕云枫,李振光,李彦阳,刘薇薇,沈明浩.白腐菌产漆酶培养条件的响应面法优化[J].安徽农业科学,2013,41(11):4701-4703,4737. |
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| 作者姓名： | 毕云枫 李振光 李彦阳 刘薇薇 沈明浩 |
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| 作者单位： | 吉林农业大学,食品质量与安全实验室,吉林长春130118;吉林农业大学,食品质量与安全实验室,吉林长春130118;吉林农业大学,食品质量与安全实验室,吉林长春130118;吉林农业大学,食品质量与安全实验室,吉林长春130118;吉林农业大学,食品质量与安全实验室,吉林长春130118 |
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| 摘 要： | 目的]采用Plackett-Burman试验设计法、响应面法,对灰盖鬼伞菌(Coprinopsis cinerea)产漆酶进行了发酵工艺条件的优化研究。方法]首先利用Plackett Burman设计试验筛选出影响产酶的麦麸、稻草粉和酵母粉3个主要因素。在此基础上,用最陡爬坡路径逼近最大响应区域,再利用Box-Behnken试验设计、响应面分析法进行回归分析。结果]麦麸、稻草粉和酵母粉浓度与漆酶活力存在显著的相关性,得到最佳培养基条件。当麦麸10.188 g/L、稻草粉9.379 g/L、酵母粉3.613 g/L时,漆酶活力达到729.89 U/ml。结论]经3批培养验证,预测值与验证试验平均值接近。
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| 关 键 词： | Coprinopsis cinerea 漆酶 培养条件 响应面法 |
Optimization of Laccase Production by Coprinopsis cinerea Using Response Surface Methodology |
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| Institution: | BI Yun-feng et al(Food Quality and Safety Laboratory,Jilin Agricultural University,Changchun,Jilin 130118) |
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| Abstract: | Objective] Process parameters for laccase production by Coprinopsis cinerea were optimized based on Plackett-Burman experimental design and response surface analysis.Method] Important factors affecting laccase production,which were identified by initial screening method of Plackett-Burman,were yeast extract,straw powder and wheat bran concentration.Path of steepest ascent was undertaken to approach the optimal region of the significant factors.Box-Behnken design and response surface analysis were adopted to further investigate mutual interaction between variables.Result] Yeast extract,straw powder and wheat bran concentration were significantly correlated with laccase production.Laccase production was up to 729.89 U/ml at the optimal conditions of yeast extract concentration 3.613 g/L,straw powder concentration 9.379 g/L and wheat bran concentration 10.188 g/L.Conclusion] After 3 times of cultivating verificataion,predication value and test value were close. |
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| Keywords: | Coprinopsis cinerea Laccase Culture condition Response surface methodology |
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