| 南瓜基因组DNA的细菌人工染色体(BAC)文库的构建 |
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| 引用本文： | 赵茜,张丽艳,徐丽珍,吴建忠.南瓜基因组DNA的细菌人工染色体(BAC)文库的构建[J].安徽农业科学,2013,41(11):4738-4739,4744. |
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| 作者姓名： | 赵茜 张丽艳 徐丽珍 吴建忠 |
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| 作者单位： | 黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨,150086;中国科学院北方粳稻分子育种联合研究中心,黑龙江哈尔滨,150086 |
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| 摘 要： | 目的]构建南瓜基因组DNA的细菌人工染色体(BAC)文库。方法]以南瓜幼芽为材料,利用HindⅢ酶切体系,初步构建南瓜基因组DNA的BAC文库。结果]研究成功构建了南瓜基因组DNA的BAC文库。结论]该技术为南瓜相关基因的克隆、功能验证、物理图谱的构建和基因组测序等研究工作奠定了基础。
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| 关 键 词： | 南瓜 基因组 BAC |
BAC Library Construction for Pumpkin Genome |
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| Institution: | ZHAO Qian et al(Industrial Crops Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086) |
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| Abstract: | Objective] The paper was to construct the bacterial artificial chromosome（BAC） library for pumpkin genomic DNA.Method] The BAC library of pumpkin genomic DNA was constructed by using pumpkin buds as materials and HindIII enzyme-cutting system.Result] The BAC Library of pumpkin genomic DNA was constructed successfully.Conclusion] The technology laid a foundation for the cloning and functional verification of related genes,physical mapping and genome sequencing of pumpkin. |
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| Keywords: | Pumpkin Genome BAC |
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