| 应用基因芯片检测中国野生葡萄抗白粉病基因表达 |
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| 引用本文： | 张军科,桑春果,王跃进,张朝红,史江莉,粟霞,罗世杏,ZHANG Jun-ke,SANG Chun-guo,WANG Yue-jin,ZHANG Chao-hong,SHI Jiang-li,SU Xia,LUO Shi-xing.应用基因芯片检测中国野生葡萄抗白粉病基因表达[J].园艺学报,2008,35(7):1053-1058. |
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| 作者姓名： | 张军科 桑春果 王跃进 张朝红 史江莉 粟霞 罗世杏 ZHANG Jun-ke SANG Chun-guo WANG Yue-jin ZHANG Chao-hong SHI Jiang-li SU Xia LUO Shi-xing |
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| 作者单位： | (西北农林科技大学园艺学院，农业部西北园艺植物种质资源与遗传改良重点开放实验室，陕西 杨凌712100) |
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| 基金项目： | 国家转基因植物研究和产业化专项基金，国家自然科学基金 |
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| 摘 要： | 本文研究了利用Affymetrix的欧洲葡萄基因表达芯片检测中国野生葡萄基因表达的可行性，并检测了葡萄白粉菌(Uncinular necator)人工接种诱导后48 h后的基因表达情况。结果表明，该芯片可以在感病材料中检测出表达的基因11 906个，抗病材料中检测到表达的基因11 839个，在二者中同时检出表达的基因11 839个，能够检出的基因数占该芯片基因探针组总数的71.3%，因此用欧洲葡萄基因芯片检测中国野生葡萄中基因表达水平是完全可行的；并利用此芯片检测出在人工接种诱导后，抗病材料中和感病材料相比表达上调的基因共1 920个，占检测出基因总数的16.21%；表达下调的基因数1 760个，占检测出基因总数的14.87%；表达上调和下调的幅度(signal log ratio)都在1~7倍之间。
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| 关 键 词： | 葡萄 基因芯片 抗病基因 基因表达 |
| 收稿时间： | 2007-12-21 |
Expression of Powdery Mildew Resistant Genes of Chinese Wild Vitis by Microarray Analysis |
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| ZHANG Jun-ke,SANG Chun-guo,WANG Yue-jin,ZHANG Chao-hong,SHI Jiang-li,SU Xia,LUO Shi-xing.Expression of Powdery Mildew Resistant Genes of Chinese Wild Vitis by Microarray Analysis[J].Acta Horticulturae Sinica,2008,35(7):1053-1058. |
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| Authors: | ZHANG Jun-ke SANG Chun-guo WANG Yue-jin ZHANG Chao-hong SHI Jiang-li SU Xia LUO Shi-xing |
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| Institution: | (College of Horticulture, Northwest A & F University, Key Laboratory of Northwest Horticultural Plant Germplasm and Genetic Improvement of Ministry of Agriculture, Yangling，Shaanxi 712100, China ) |
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| Abstract: | In this paper, the feasibility of using Affymetrix Vitis vinifera gene expression microarray to detect the gene expression of Chinese wild grape was studied, and the gene expression 48 h after artificial inoculation with Uncinular necator was detected. The results showed that the microarray can detect the expression of 11 906 genes in susceptible material (S), while 11 839 in resistant individual (R) and in both R and S 11 839 genes. The detected genes accounted for ~71.3% of the total 16 602 probe groups. That means Affymetrix V. vinifera microarray can be efficiently used for gene expression analysis of Chinese wild grapes. Compared to the S plant, 1 920 up regulated and 1 760 down regulated genes were detected in R plant, accounting for 16.21% and 14.87% of total detected genes respectively, up and down regulated rate (signal log ratio) are between 1~7. |
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| Keywords: | grapevine gene microarray disease resistance gene gene expression |
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