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Ca2+对皖贝母高温胁迫下抗逆生理指标及光合作用的影响
引用本文:李同根,王康才,罗庆云,李丹霞.Ca2+对皖贝母高温胁迫下抗逆生理指标及光合作用的影响[J].植物营养与肥料学报,2012,18(3):765-770.
作者姓名:李同根  王康才  罗庆云  李丹霞
作者单位:1.南京农业大学中药材研究所,南京mily
基金项目:深圳市三九现代中药有限公司项目(2010126)资助
摘    要:分析Ca2+对高温胁迫下皖贝母抗逆生理指标及相关光合参数的影响,探讨钙对皖贝母高温胁迫伤害调控的可行性。将室外同一环境条件下盆栽的皖贝母,分别叶面喷施5、10、20、30、50 mg/L的CaCl2溶液,然后置于35℃/25℃(昼/夜),光强3 600 lx的光照培养箱内,进行高温胁迫处理,以常温25℃喷施蒸馏水和高温胁迫下喷施蒸馏水为对照,处理3 d后,测定皖贝母叶片相关光合参数、膜透性、脯氨酸含量、可溶性蛋白含量及SOD、POD活性。结果表明:高温胁迫下叶面喷施CaCl2溶液,可增强皖贝母叶片SOD、POD酶活性,提高叶片游离脯氨酸及可溶性蛋白含量,有效减少高温胁迫对细胞膜的破坏,显著降低相对电导率。同时,Ca2+处理提高了叶片中叶绿素与类胡萝卜素含量,提高了叶片光合效率。结论:叶面喷施一定浓度CaCl2溶液可减轻高温胁迫对皖贝母叶片的伤害。

关 键 词:皖贝母    Ca2+    高温胁迫    光合作用
收稿时间:2011-09-19

Effects of exogenous Ca2+ on physiological and photosynthesis of Fritillaria anhuiensis under high temperature stress
LI Tong-gen,WANG Kang-cai,LUO Qing-yun,LI Dan-xia.Effects of exogenous Ca2+ on physiological and photosynthesis of Fritillaria anhuiensis under high temperature stress[J].Plant Nutrition and Fertilizer Science,2012,18(3):765-770.
Authors:LI Tong-gen  WANG Kang-cai  LUO Qing-yun  LI Dan-xia
Institution:1.Institute of Chinese Medicinal Materials,Nanjing Agricultural University,
Abstract:In order to reveal feasibility of the regulation of Ca2+ on Fritillaria anhuiensis under high temperature stress,the effects of Ca2+ on physiological index and related photosynthetic parameters were studied.Pot cultured Fritillaria anhuiensis leaves under the same outdoor conditions were sprayed with 5,10,20,30 and 50 mg/L CaCl2 separately,and then placed in the light incubator 35℃/25℃(day/night),light intensity 3 600 lx],and sprayed with distilled water at 25℃ and under high temperature stress were set as the control.After treated for 3 days,related photosynthesis,relative conductivity,contents of proline and soluble protein,activities of SOD and POD were tested.The results show that the exogenous Ca2+ enhances the activities of SOD and POD,the contents of proline and soluble protein,and effectively reduces the damage of heat stress on cell membrance.At the same time,the exogenous Ca2+ increases the contents of chlorophyll and carotenoid,the efficiency of leaf photosynthesis.In conclusion,the foliage spraying CaCl2 could reduce the damage of high temperature stress on Fritillaria anhuiensis leaves.
Keywords:Fritillaria anhuiensis  Ca2+  high temperature stress  photosynthesis
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