Identification of <Emphasis Type="Italic">CpTI</Emphasis> gene integration for 2-year-old transgenic poplars at DNA level |
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| Authors: | Zhang?Qian Lin?Shanzhi Lin?Yuanzhen Email author" target="_blank">Zhang?Zhiyi?Email author Liu?Haijun Zou?Yening Wang?Zeliang |
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| Institution: | (1) College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083 Beijing, P. R. China;(2) College of Resources and Environment, Beijing Forestry University, 100083 Beijing, P. R. China |
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| Abstract: | The putative transgenic hybrid triploid poplars (P. tomentosa × P. bolleana) × P. tomentosa] with CpTI gene have been outplanted in test field for 2 years. Although the authors’ previous studies have proved that they are highly
resistant to 3 species of poplar-threatening insect pests and contain high content of CpTI protein in foliage, incorporation status of foreign CpTI gene in poplar genome is uncertain. In this present study, the incorporation of foreign CpTI gene in genome of 5 transgenic poplars was confirmed by PCR and Southern blotting analysis. DNA amplification showed that
there were clear DNA bands of about 450bp specific to CpTI gene in transgenic lanes, while no corresponding band in non-transgenic lane was observed. Correspondingly, clear DNA hybridization
signals and no signal were exhibited on film for DNA Southern blotting analysis in transgenic lanes and non-transgenic lane,
respectively, which further confirmed the stable integration of foreign CpTI gene in genome of 2-year-old transgenic poplar.
Supported by the National Project in Transgenic Plant and Application (Grant No. J2002-B003)] |
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| Keywords: | cowpea trypsin inhibitor Populus tomentosa DNA PCR Southern blotting |
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