| 鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析(英文) |
| |
| 引用本文： | 皇甫和平,王川庆,陈陆,杨霞,刘红英,彭志锋,郑鹿平,徐雪,刘慧敏,付仁一,郭伦涛.鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析(英文)[J].Agricultural Science&Technology,2009(1). |
| |
| 作者姓名： | 皇甫和平 王川庆 陈陆 杨霞 刘红英 彭志锋 郑鹿平 徐雪 刘慧敏 付仁一 郭伦涛 |
| |
| 作者单位： | 河南农业大学牧医工程学院;郑州牧业工程高等专科学校; |
| |
| 基金项目： | Supported by International Collaboration Program (43006167)~~ |
| |
| 摘 要： | Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus-specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; the highest identity (99.9%) was observed between the isolated strain and one of the strains of Gallibacterium anatis (AF228002), the homologies between the isolated strain and 3 strains of gallibacterium accessed in NCBI (AF228016, Gallibacterium genomosp.1, AF228017, Gallibacterium genomosp.2, AF228018, Gallibacterium genomosp.1) were above 97.1%, higher than that of the isolated strain and the other strains of the other 11 genera which were between 90.7%-93.2%. It can be seen from the phylogenetic tree that the 2 isolated strains and the other 4 strain of gallibacterium fell into the same branch, furthermore the 2 isolated strains and the strain of Gallibacterium anatis locate in an internal branch, indicating that the 2 isolated strains belong to Gallibacterium anatis.
|
| 关 键 词： | 特异性 巴氏杆菌 分离株 细菌分离培养 卡氏 同源性分析 输卵管炎 分离菌株 系统发育树 大肠杆菌 |
Molecular Identification of Gallibacterium from Hen and Analysis of Its 16S rRNA Gene |
| |
| HUANGFU He-ping,WANG Chuan-qing,CHEN Lu,YANG Xia,LIU Hong-ying,PENG Zhi-feng,ZHENG Lu-ping,XU Xue,LIU Hui-min,FU Ren-yi,GUO Lun-tao.College of Animal Husb,ry , Veterinary Medicine,Henan Agricultural University,Zhengzhou ,.Zhengzhou College of Animal Husb,ry Engineering,Zhengzhou.Molecular Identification of Gallibacterium from Hen and Analysis of Its 16S rRNA Gene[J].农业科学与技术(英文版),2009(1). |
| |
| Authors: | HUANGFU He-ping WANG Chuan-qing CHEN Lu YANG Xia LIU Hong-ying PENG Zhi-feng ZHENG Lu-ping XU Xue LIU Hui-min FU Ren-yi GUO Lun-taoCollege of Animal Husb ry Veterinary Medicine Henan Agricultural University Zhengzhou Zhengzhou College of Animal Husb ry Engineering Zhengzhou |
| |
| Institution: | HUANGFU He-ping1,2,WANG Chuan-qing1,CHEN Lu1,YANG Xia1,LIU Hong-ying1,PENG Zhi-feng1,ZHENG Lu-ping1,XU Xue1,LIU Hui-min1,FU Ren-yi1,GUO Lun-tao11.College of Animal Husb,ry , Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,2.Zhengzhou College of Animal Husb,ry Engineering,Zhengzhou 450011 |
| |
| Abstract: | Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus-specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; ... |
| |
| Keywords: | Hen Gallibacterium Isolation and identification Homology analysis |
| 本文献已被 CNKI 等数据库收录！ |
|
