Preliminary characterization of race-specific elicitors from<Emphasis Type="Italic">Peronospora parasitica</Emphasis> and their ability to elicit phenolic accumulation in<Emphasis Type="Italic">Arabidopsis</Emphasis> |
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| Authors: | Email author" target="_blank">S?SoyluEmail author E?M?Soylu |
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| Institution: | (1) Dept. of Plant Protection, Faculty of Agriculture, University of Mustafa Kemal, 31034 Antakya, Hatay, Turkey |
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| Abstract: | Intercellular washing fluid (IWF) obtained from the susceptibleArabidopsis accession Ws-eds1 inoculated withPeronospora parasitica isolate Emoy-2, contained an elicitor of necrosis with ecotype specificity towardsArabidopsis accessions with particular resistance genes. This elicitor caused necrosis on the highly resistant accessions La-er, Nd-1
and partly on Col-5, but not on the susceptible accessions Ws-eds1 and Oy-0. In resistant plants, injection of IWF caused hypersensitive reaction (HR)-like cell collapse which was associated
with the accumulation of phenolics and lignin-like material in walls of cells undergoing cell death. The elicitor is sensitive
to proteinase K and pronase enzymes, heating and autoclaving but insensitive to periodate oxidation, freezing and thawing,
and is not dialyzable. Results suggest that the elicitor is a protein. Fractionation experiments using size-exclusion membranes
revealed that elicitor activity has a molecular weight in excess of 100 kDa.
http://www.phytoparasitica.org posting July 13, 2003. |
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| Keywords: | Race-specific elicitor Arabidopsis Peronospora resistance |
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