| Comparative Proteomic Analysis Shows an Elevation of Mdh1 Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats |
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| 基金项目： | Acknowledgements This work was supported by the Project of China Agriculture Research System （CARS-40-30）. |
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| 摘 要： | Copper nanoparticle is a new material widely used in biological medicine, animal husbandry and industrial areas, but its potential toxicity to human health and environment remains unclear. In order to study the hepatotoxic mechanisms of nanoparticles copper, two-dimensional gel electrophoresis （2-DE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI/TOF MS） of proteomics technology were used to isolate and identify the differentially expressed proteins from liver, which associated with hepatotoxicity induced by copper nanoparticle in rats. In this study, we have screened 15 kinds of proteins related with hepatotoxicity, of which spot8212 was identified as Malate dehydrogenase （Mdhl）. The mRNA expression trend of Mdhl was consistent with the result of 2-DE by RT-PCR validation. Bioinformatics analysis showed that Mdhl was stable and no signal peptides, subcellular location was in endoplasmic reticulum; it contained many functional sites such as malate dehydrogenase activity signal sites 155LTRLDHNRAKSQI167; a helixes and random coils were the two main elements. Homologous analysis demonstrated high homologous of Mdhl in rats with mouse and human, and the phylogenetic tree of Mdhl was constructed. The result indicated that copper nanoparticle could regulate up the Mdhl protein expression so as to compensate the energy deficit. Energy metabolic disturbance may be a pathway for copper nanoparticle particles to exert the hepatotoxic effects in rats.
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| 关 键 词： | 铜纳米粒子 肝毒性 蛋白质组分 大鼠 诱导 基质辅助激光解吸 苹果酸脱氢酶 蛋白质组学技术 |
Comparative Proteomic Analysis Shows an Elevation of Mdhl Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats |
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| Authors: | DONG Shu-wei ;GAO Zhao-hui ;SHEN Xiao-yun ;XUE Hui-wen ;LI Xia |
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| Institution: | [1]Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture~Key Laboratory of New Animal Drug Project, Gansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, P.R.China; [2]Bijie College, Bijie 551700, P.R.China; [3]College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R. China |
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| Abstract: | copper nanoparticle, hepatotoxicity, proteomics, malate dehydrogenase, bioinformatics, rat |
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| Keywords: | copper nanoparticle hepatotoxicity proteomics malate dehydrogenase bioinformatics rat |
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