| Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine |
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| 基金项目： | Acknowledgements The authors would like to acknowledge the Key Laboratory of Animal Parasitology of the Ministry of Agriculture for providing the laboratory equipments used in this study. This work was funded by the National Key Technology R＆D Program, China （2008 BADB4B05-02）. |
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| 摘 要： | Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography （HPLC） fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharrnacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.
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| 关 键 词： | 色谱指纹图谱 高效液相色谱法 活性物质 中国医药 抗球虫 光谱效应 HPLC指纹图谱 生物活性成分 |
Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine |
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| Authors: | XIAO Sui FEI Chen-zhong ZHANG Li-fang ZHENG Wen-li ZHANG Ke-yu XUE Fei-qun |
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| Institution: | Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy ofAgricultural Sciences, Shanghai 200241, P.R. China |
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| Abstract: | herb medicine, fingerprint, anticoccidial index, spectrum-effect relationship |
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| Keywords: | herb medicine fingerprint anticoccidial index spectrum-effect relationship |
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