| 激素组合与水解蛋白对番茄花药愈伤增殖的影响 |
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| 引用本文： | 蒋青青,刘杨,陈火英.激素组合与水解蛋白对番茄花药愈伤增殖的影响[J].上海交通大学学报(农业科学版),2012,30(2):8-11,59. |
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| 作者姓名： | 蒋青青 刘杨 陈火英 |
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| 作者单位： | 上海交通大学农业与生物学院,上海,200240 |
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| 基金项目： | 国家863计划，上海市科技兴农重点攻关项目，上海市重点学科建设项目 |
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| 摘 要： | 以4个番茄F1杂交种(C1、C2、C8、C10)和1个番茄纯系(2052)花药培养的愈伤组织为材料,研究了不同激素组合对愈伤组织增殖的影响,以及不同浓度水解酪蛋白(CH)和水解乳蛋白(LH)对愈伤组织增殖的影响。结果表明:MS(NLN)+1.00mg/L IAA+1.00mg/L 6-BA+30g/L蔗糖和MS(NLN)+0.50mg/L NAA+0.50mg/L KT+30g/L蔗糖这2种激素组合的培养基中愈伤组织生长最快,其中材料C8的增殖系数最高达0.61;愈伤组织增殖最适浓度为5.00g/L CH和0.50g/L LH,且在5个材料中C8愈伤增殖系数最大,分别为1.00和0.91。
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| 关 键 词： | 番茄 花药培养 水解酪蛋白(CH) 水解乳蛋白(LH) |
Effects of Casein Hydrolysate,Lactoalbumin Hydrolysate and Hormones Combination on Tomato Anther Callus Propagation |
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| JIANG Qing-qing
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LIU Yang
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CHEN Huo-ying.Effects of Casein Hydrolysate,Lactoalbumin Hydrolysate and Hormones Combination on Tomato Anther Callus Propagation[J].Journal of Shanghai Jiaotong University (Agricultural Science),2012,30(2):8-11,59. |
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| Authors: | JIANG Qing-qing LIU Yang CHEN Huo-ying |
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| Institution: | (School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Four F1 hybrids(C1,C2,C8,C10) and one inbred line(2052) were used to investigate the effect of different kinds of hormones combination,different concentration of Casein Hydrolysate(CH) and Lactoalbumin Hydrolysate(LH) on callus propagation.The results showed that the optimum mediums for callus propagation were MS(NLN) + 1.00 mg/L IAA + 1.00 mg/L 6-BA + 30 g/L sugar and MS(NLN) + 0.50 mg/L NAA + 0.50 mg/L KT + 30 g/L sugar,and the highest propagation index was 0.61 in C8.The preferred concentrations for callus propagation were 5.00 g/L of CH and 0.50 g/L of LH.The propagation index in C8 were 1.00 and 0.91,respectively. |
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| Keywords: | tomato anther culture Casein Hydrolysate(CH) Lactoalbumin Hydrolysate(LH) |
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