Analysis of radiation-induced W chromosome aberrations in the codling moth, <Emphasis Type="Italic">Cydia pomonella</Emphasis> (L.), by fluorescence in situ hybridization techniques |
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| Authors: | H Makee N Tafesh F Marec |
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| Institution: | (1) Atomic Energy Commission, P.O. Box 6091, Damascus, Syria;(2) Biologicy Centre, ASCR, Institute of Entomology, and Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic |
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| Abstract: | The structure of the female sex-determining W chromosome was examined in wild-type females and in three mutant female lines
of the codling moth, Cydia pomonella. The mutant lines were isolated from progeny of irradiated females using the abnormal appearance of sex chromatin body in
female somatic polyploid nuclei as a cytogenetic marker. A detailed cytogenetic analysis was carried out using three different
fluorescence in situ hybridization techniques: genomic in situ hybridization (GISH), comparative genomic hybridization (CGH),
and fluorescence in situ hybridization (FISH) with the codling moth W-chromosome painting probe. The FISH techniques enabled
us to identify the W chromosome and its fragments in mitotic and meiotic chromosome complements and thus helped us to resolve
complicated W chromosome aberrations induced by irradiation. |
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| Keywords: | Codling moth W chromosome W-chromosome painting probe Genomic hybridization CGH FISH GISH Radiation-induced aberrations |
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