| 利用RAPD引物对四川乳肉兼用牛及其父母本的遗传距离鉴定 |
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| 引用本文： | 曹冶,王淮,赵素君,谢晶,付茂忠,易礼胜,赵益元.利用RAPD引物对四川乳肉兼用牛及其父母本的遗传距离鉴定[J].西南农业学报,2009,22(5). |
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| 作者姓名： | 曹冶 王淮 赵素君 谢晶 付茂忠 易礼胜 赵益元 |
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| 作者单位： | 1. 四川省畜牧科学研究院,四川,成都,610066
2. 宣汉县畜牧局,四川,宣汉,636150 |
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| 基金项目： | 四川省育种攻关项目:牛新品系选育 |
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| 摘 要： | 为了鉴定四川乳肉兼用牛与父母本的亲缘关系,从50条随机引物中筛选出6条引物对四川乳肉兼用牛及其父母本进行扩增.6条RAPD引物共扩增出334个条带.将这些多态性条带进行聚类分析,结果显示:四川乳肉兼用牛与3个父母本品种牛的遗传差距并不大,在0.01～0.25之间,其中与西门塔尔牛遗传距离最近,与宣汉本地黄牛的遗传距离最远.这与传统的形态学结果一致.
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| 关 键 词： | 四川乳肉兼用牛 鉴定 随机引物扩增多态性 |
Identification of Sichuan Cattle and Its Parents by RAPD Primers |
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| CAO Ye,WANG Huai,ZHAO Su-jun,XIE Jing,FU Mao-zhong,YI Li-sheng,ZHAO Yi-yuan.Identification of Sichuan Cattle and Its Parents by RAPD Primers[J].Southwest China Journal of Agricultural Sciences,2009,22(5). |
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| Authors: | CAO Ye WANG Huai ZHAO Su-jun XIE Jing FU Mao-zhong YI Li-sheng ZHAO Yi-yuan |
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| Abstract: | To identify the relationship of the Sichuan cattle and its parents, 6 primers were screened from the 50 random primers to amplify the Sichuan cattle and its parents.334 bands were amplified.Cluster analysis results displayed that the genetic background among the 4 cattle species were not large,between 0.01-0.25,and the genetic distance between the Sichuan cattle and Simmental cattle was the nearest ,that between the Sichuan cattle and the Xuanhan's local cattle was the farthest.This was coincidence with the traditional morphological result. |
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| Keywords: | Sichuan cattle Identification RAPD |
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