| 利用分子标记辅助选择改良温敏核不育系GD-8S的稻瘟病抗性 |
| |
| 引用本文： | 金素娟,柳武革,朱小源,王丰,李金华,刘振荣,廖亦龙,朱满山,黄慧君,刘宜柏.利用分子标记辅助选择改良温敏核不育系GD-8S的稻瘟病抗性[J].中国水稻科学,2007,21(6):599-604. |
| |
| 作者姓名： | 金素娟 柳武革 朱小源 王丰 李金华 刘振荣 廖亦龙 朱满山 黄慧君 刘宜柏 |
| |
| 作者单位： | 1.广东省农业科学院 水稻研究所， 广东 广州 510640； 2江西农业大学 农学院， 江西 南昌 330045； 3广东省农业科学院 植物保护研究所， 广东 广州 510640； |
| |
| 基金项目： | 国家高技术研究发展计划(863计划);广东省科技专项基金;广东省农业攻关资助 |
| |
| 摘 要： | 以含有广谱稻瘟病抗性基因Pi 1的籼稻材料BL122为供体，优质感病温敏核不育系GD 8S为受体，通过杂交、多次回交和自交结合分子标记辅助选择技术，将Pi 1基因导入GD 8S中。综合目标基因的分子检测、育性镜检、田间农艺性状表现以及遗传背景的回复率，筛选获得5个改良株系RGD8S 1、RGD8S 2、RGD8S 3、RGD8S 4和RGD8S 5。采用广东省具有代表性的33个菌株进行人工接种抗性鉴定，5个改良株系的抗性频率为75.76%~100.00%，而对照仅为9.09%。农艺性状分析表明，除剑叶长和每株穗数外，其余性状改良株系与对照无显著差异。这些改良株系可用于进一步测配选育高抗稻瘟病杂交稻新组合。
|
| 关 键 词： | 稻瘟病抗性 温敏核不育系 分子标记辅助选择 育种 水稻 |
| 文章编号： | 1001-7216（2007）06-0599-06 |
| 收稿时间： | 2007-03-30 |
| 修稿时间： | 2007-06-08 |
Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Line GD-8S by Molecular Marker-Assisted Selection |
| |
| JIN Su-juan,LIU Wu-ge,ZHU Xiao-yuan,WANG Feng,LI Jin-hua,LIU Zhen-rong,LIAO Yi-long,ZHU Man-shan,HUANG Hui-jun,LIU Yi-bai.Improving Blast Resistance of a Thermo-Sensitive Genic Male Sterile Line GD-8S by Molecular Marker-Assisted Selection[J].Chinese Journal of Rice Science,2007,21(6):599-604. |
| |
| Authors: | JIN Su-juan LIU Wu-ge ZHU Xiao-yuan WANG Feng LI Jin-hua LIU Zhen-rong LIAO Yi-long ZHU Man-shan HUANG Hui-jun LIU Yi-bai |
| |
| Institution: | 1Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China ; 2 Agronomy College, J iangxi Agri cultural University, Nanchang 330045, China ; 3 Plant Protection Research Institute, Ouangdong Academy of Agricultural Sciences, Ouang zhou 510640, China; |
| |
| Abstract: | The broad-spectrum blast resistance gene Pi-1,from the donor line BL122,was introduced into a thermo-sensitive genic male sterile(TGMS)line GD-8S,which possessed good grain quality but high susceptibility to rice blast,by using backcross breeding and molecular marker-assisted selection.Five elite improved male sterile lines RGD8S-1,RGD8S-2,RGD8S-3,RGD8S-4 and RGD8S-5,were selected based on the results of molecular marker analysis,spikelet sterility,recovery ratio of genetic background and agronomic traits.Thirty-three representative blast isolates collected from Guangdong Province,China were used to inoculate the improved lines and the origin line GD-8S artificially.The resistant frequency of the improved lines ranged from 75.76% to 100.00%,much higher than that of the check GD-8S,which was only 9.09%.On the agronomic characters,there was no significant differences between the improved lines and the check GD-8S except for flag leaf length and panicle number per plant.The improved lines could be used for breeding hybrid rice with high blast resistance. |
| |
| Keywords: | blast resistance thermo-sensitive genic male sterile line molecular marker-assisted selection breeding rice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国水稻科学》浏览原始摘要信息 |
| 点击此处可从《中国水稻科学》下载免费的PDF全文 |
|
