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| 应用原核表达的猪圆环病毒Ⅱ型Cap蛋白建立一种间接ELISA诊断方法 | |
| 引用本文： | 林彦星,孙彦伟,刘镇明,陈靳松,龚善中,徐铮.应用原核表达的猪圆环病毒Ⅱ型Cap蛋白建立一种间接ELISA诊断方法[J].中国预防兽医学报,2007,29(3):217-221. |
| 作者姓名： | 林彦星 孙彦伟 刘镇明 陈靳松 龚善中 徐铮 |
| 作者单位： | 1. 华南农业大学,兽医学院,广东,广州,510640 2. 广东省,动物防疫监督总所,广东,广州,510230 3. 华南农业大学,动物科学学院,广东,广州,510640 |
| 摘 要： | 本试验在最佳诱导条件下获得猪圆环痛毒Ⅱ型重组Cap蛋白的基础上，利用HisBind蛋白质纯化试剂盒对表达产物进行纯化，通过Westernblot检测证明表达产物具有良好的抗原性。以纯化后的重组融合蛋白作为诊断抗原，对ELISA反应条件进行优化，初步建立了检测PCV2抗体的间接ELISA方法。用该法对广东、广西一些地区收集到的380份血清样品进行检测，检测结果阳性率为86．1％，从中随机取90份猪血清样品与国产商品化试剂盒检测结果对比，符合率为95．6％，表明本实验建立的间接ELISA方法具有较高的敏感性和特异性，适于大规模检测PCV2血清抗体的流行病学调查。 |
| 关 键 词： | 重组Cap蛋白 表达 纯化 |
| 文章编号： | 1008-0589（2007）03-0217-05 |
| 收稿时间： | 2006-06-16 |
| 修稿时间： | 2006年6月16日 |
Development of an indirect ELISA diagnostic method with the main antigentic region of PCV2 capsid protein expressed in E.coli |
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| LIN Yan-xing,SUN Yan-wei,LIU Zhen-ming,CHEN Jin-song,GONG Shan-zhong,XU Zheng.Development of an indirect ELISA diagnostic method with the main antigentic region of PCV2 capsid protein expressed in E.coli[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(3):217-221. | |
| Authors: | LIN Yan-xing SUN Yan-wei LIU Zhen-ming CHEN Jin-song GONG Shan-zhong XU Zheng |
| Abstract: | An indirect enzyme-linked immunosorbent assay(ELISA) was developed based on a recombinant capsid protein of porcine circovirus type 2(PCV2).The recombinant capsid protein was expressed under the optimum induced condition,purified by a His Bind Purification Kit,and confirmed by Western blot assay.The indirect ELISA was used to test against 380 serum samples collected from Guangdong and Guangxi province and 86.1 % samples were positive.Compared with commercial ELISA kits,the assay showed a correlation rate of 95.6 % based on the data obtained from 90 randomly picked serum samples.The results indicated that the indirect ELISA is not only sensitive and specific,but also suitable for large-scale epidemiological investigation for PCV2 infection. |
| Keywords: | PCV2 ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |