SSR and SCAR markers linked to the fertility-restoring gene for a D2-type cytoplasmic male-sterile line in wheat |
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| Authors: | X L Li L K Liu N Hou G Q Liu C G Liu |
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| Institution: | State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;Graduate School of Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | ‘Yi 4060’ is an elite restorer line of a non‐photoperiod‐sensitive D2‐type cytoplasmic male‐sterile (CMS) line of wheat. Random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were employed to map one major fertility‐restoring gene (D2Rf1) in ‘Yi 4060′. The sterile and fertile DNA pools were established from individuals in BC6, based on bulked segregant analysis. One RAPD marker E09, linked to D2Rf1, was converted to a SCAR marker and designated as E09‐SCAR865. The genetic distance between E09‐SCAR865 and D2Rf1 is 9.5 cM. Two SSR markers, Xgwm11 and Xgwm18, were also linked to a D2Rf1 and co‐segregated with E09‐SCAR865. The three molecular markers are useful in marker‐assisted breeding of the elite restorer lines for D2 ‐type CMS lines in wheat. |
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| Keywords: | Triticum aestivum cytoplasmic male sterility fertility-restoring gene SCAR SSR |
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