| 皱纹盘鲍血细胞活性氧产生的研究↑（*） |
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| 引用本文： | 张峰,李光友,张培军.皱纹盘鲍血细胞活性氧产生的研究↑（*）[J].中国水产科学,1999,6(3):36-40. |
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| 作者姓名： | 张峰 李光友 张培军 |
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| 作者单位： | 大连水产学院养殖系!大连116023(张峰)，国家海洋局第一海洋研究所!青岛266003(李光友)，中国科学院海洋研究所!青岛266071(张培军) |
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| 基金项目： | 国家攀登计划项目!PD6 -6 -3 |
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| 摘 要： | 在体外条件下用不同刺激物(海洋酵母细胞和酵母聚糖)对皱纹盘鲍血细胞进行刺激,测定血细胞吞噬活动中经历呼吸爆发产生活性氧的化学发光反应。结果表明,皱纹盘鲍血细胞经刺激诱导吞噬活动中有明显的呼吸爆发现象和很强的活性氧产生。不同的刺激物产生的化学发光强度不同;同一种刺激物的不同处理和不同浓度对血细胞产生活性氧的化学发完强度的影响不同。刺激物经皱纹盘鲍自体血清调理和未经调理对血细胞刺激所产生的化学发光强度不同。SOD和NaN3对血细胞吞噬过程中活性氧产生的化学发光有抑制作用。皱纹盘鲍血细胞在吞噬防御反应中能够通过产生活性氧对外源病原进行杀灭。
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| 关 键 词： | 皱纹盘鲍 血细胞 活性氧 吞噬作用 呼吸爆发 化学发光 |
| 修稿时间： | 2010/4/8 0:00:00 |
Study on the gerneration of reactive oxygen species by hemocytes in Haliotis discus |
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| Zhang Feng,Li Guangyou.Study on the gerneration of reactive oxygen species by hemocytes in Haliotis discus[J].Journal of Fishery Sciences of China,1999,6(3):36-40. |
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| Authors: | Zhang Feng Li Guangyou |
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| Abstract: | The hemocytes of Haliotis discus are stimulated in vitro with various particulate agents (yeast cells and zymosan)so as to observe the chemiluminescence (CL) caused by the reactive oxygen species (ROS). The results indicate that the hemocytes of H. discus will undergo an obvious burst of respiratory and generate severe active oxygen when they are stimulated in vitro to begin their phagocytic activity. With different stimulators, or with the same stimulator under its different treatment and concentrations, the hemocytes show different degree of CL, also, they generate different degree of CL with yeast cells opsonized or nonopsonized by the H. disscus serum. SOD and NaN 3 can inhibit the CL caused by ROS during the phagocytosis. It comes to the conclusion that the hemocytes of H. discus can generate ROS to kill their pathogens in the phagocytic defensive reaction. |
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| Keywords: | Haliotis discus hemocyte reactive oxygen species (ROS) phagocytosis respiratory burst chemiluminescence |
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