| PRRSV高致病性毒株RT-PCR鉴别诊断方法的建立 |
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| 引用本文： | 赵泽坤,王娇,王金凤,张岭岭,孙继国.PRRSV高致病性毒株RT-PCR鉴别诊断方法的建立[J].广东畜牧兽医科技,2010,35(2):36-38. |
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| 作者姓名： | 赵泽坤 王娇 王金凤 张岭岭 孙继国 |
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| 作者单位： | 河北农业大学动物科技学院,河北,保定,071001 |
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| 摘 要： | 根据GenBank中已发表的PRRSV的NSP2基因序列,设计一对特异性引物。针对经典型毒株与高致病性毒株,采用RT-PCR方法扩增,可分别扩增出相应的576bp,486bp的目的片段。从而在检测PRRSV的基础上,进一步区分PRRSV的经典型和高致病性毒株。通过对扩增条件的筛选,成功地建立了猪繁殖与呼吸综合征经典型与高致病性毒株的RT-PCR鉴别诊断方法,且为PRRSV分离株的鉴定分型及NSP2功能研究奠定了基础。
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| 关 键 词： | 猪繁殖与呼吸综合征病毒 NSP2 RT-PCR 鉴别诊断 |
Development of a RT-PCR method for the differention and identification of the highly pathogenic PRRSV |
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| Zhao Zekun,Wang Jiao,Wang Jinfeng,Zhang Lingling,Sun Jiguo.Development of a RT-PCR method for the differention and identification of the highly pathogenic PRRSV[J].Guangdong Journal of Animal and Veterinary Science,2010,35(2):36-38. |
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| Authors: | Zhao Zekun Wang Jiao Wang Jinfeng Zhang Lingling Sun Jiguo |
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| Institution: | College of Animal Science and Technology;Agricultural University of Hebei;Baoding 071001;China |
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| Abstract: | A pair of primers was designed based on the NSP2 sequences of PRRSV published in GenBank for the differention and identification of classical PRRSV strains and highly pathogenic PRRSV strains.Using RT-PCR method a fragment of 576bp or 486bp could be amplified for classical PRRSV strains or highly pathogenic PRRSV strains respectively.So the differention and identification of classical PRRSV strains and highly pathogenic PRRSV strains could be done.The RT-PCR method lay a foundation for the differention and ... |
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| Keywords: | Porcinereproductiveandrespiratorysyndrome NSP2 RT-PCR differentionandidentification |
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