| 植物乳杆菌C88胞外多糖生物合成基因的克隆及序列比对 |
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| 引用本文： | 赵玉娟,李盛钰,张莉,牛春华,张雪,李达,张贵斌,杨贞耐.植物乳杆菌C88胞外多糖生物合成基因的克隆及序列比对[J].广西农业生物科学,2011,30(4):331-337. |
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| 作者姓名： | 赵玉娟 李盛钰 张莉 牛春华 张雪 李达 张贵斌 杨贞耐 |
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| 作者单位： | 1. 吉林省农业科学院农产品加工研究中心,长春,130033
2. 长春新高食品有限公司,长春,130103 |
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| 基金项目： | 国家自然科学基金项目，农业部现代农业产业技术体系建设专项资金资助项目，吉林省博士后基金项目 |
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| 摘 要： | 乳酸菌胞外多糖能显著改善发酵乳制品及食品的流变学和质构特性。为进一步了解乳酸菌胞外多糖的生物合成途径及调控机制，本研究对参与植物乳杆菌C88胞外多糖生物合成基因簇的部分序列进行了克隆和鉴定。根据GenBank中己报道植物乳杆菌基因序列的保守区域设计特异性引物，扩增出植物乳杆菌C88生物合成蛋白基因（cps4A）序列，并通过染色体步移方法克隆了植物乳杆菌C88参与胞外多糖合成基因簇的部分序列（4．9kb）。利用生物信息学方法预测基因簇中6个阅读框的结构和功能，结果表明该序列与己报道的乳酸杆菌胞外多糖生物合成基因具有高度的同源性（〉96％）；对各阅读框功能预测分析发现，这6个基因主要编码参与胞外多糖合成中的多糖合成蛋白、糖链长度检测蛋白、UDP-葡萄糖-4-异构酶和糖基转移酶。本研究将为利用基因工程方法调控多糖的合成和产量提供理论依据。
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| 关 键 词： | 植物糖乳杆菌 胞外多糖 基因簇 生物合成 |
Cloning and Sequence Alignment of Exopolysaccharide Biosynthesis Genes of Lactobacillus plantarum C88 |
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| Zhao Y ujuan,Li Shengyu,Zhang Li,Niu Chunhua,Zhang Xue,Li Da,Zhang Guibin,Yang Zhennai.Cloning and Sequence Alignment of Exopolysaccharide Biosynthesis Genes of Lactobacillus plantarum C88[J].Journal of Guangxi Agricultural and Biological Science,2011,30(4):331-337. |
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| Authors: | Zhao Y ujuan Li Shengyu Zhang Li Niu Chunhua Zhang Xue Li Da Zhang Guibin Yang Zhennai |
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| Institution: | Zhao Y ujuan Li Shengyu Zhang Li Niu Chunhua Zhang Xue Li Da Zhang Guibin Yang Zhennai ( 1 Center of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun, 130033; 2 School of Biological and Agricultural Engineering, Jilin University, Chuangchun, 130022; 3 Changchun Xingao Food Co., Ltd, Changchun, 130103) |
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| Abstract: | Exopolysaccharides （EPS） produced by lactic acid bacteria （LAB） are used to generate the rheological characteristics and texture properties of specific fermented dairy and food products. To better understand the EPS biosynthetic pathway and mechanism, some genes involved EPS biosynthesis ofLactobacillus plantarum C88 were coloned and identified in this paper. Specific primer was designed according to the conservative gene sequences of Lactobaeillus plantarum strains from GenBank. Exopolysaccharide （EPS） biosynthesis protein （cps4A） of L. plantarum C88 was amplified by PCR, and 4.9 kb region consisting of 6 open reading frames （ORFs） was acquired by chromosome walking. The results of bioinformatics analysis showed that the predicted protein products of the six ORFs had high homology with the EPS biosynthetic genes oflactobacilli （homology〉96%）. Functions were assigned to some of the predicted gene products on the basis of homology to known sequences as follows： The genes cps4A, cps4B, cps4C, cps4E, cps4F and galE3 mainly encode putative EPS biosynthesis protein, chain-length determinant protein, glycosyltransferase and UDP-glucose 4~epimerase, respectively. The results of this study would provide a foundation for further understanding the EPS biosynthetic pathway of L. plantarum and its related mechanism. |
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| Keywords: | Lactobacillus plantarum Exopolysaccharide Gene cluster Biosynthesis |
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