| 晋麦F2籽粒中HMW-GS的遗传规律研究 |
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| 引用本文： | 张丽,张建刚,潘登奎.晋麦F2籽粒中HMW-GS的遗传规律研究[J].安徽农业科学,2009,35(19):8918-8919. |
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| 作者姓名： | 张丽 张建刚 潘登奎 |
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| 作者单位： | 山西农业大学文理学院,山西太谷,030801;山西农业大学文理学院,山西太谷,030801;山西农业大学文理学院,山西太谷,030801 |
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| 基金项目： | 山西农业大学青年基金 |
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| 摘 要： | 目的]为杂种小麦加工品质预测和优质小麦亲本组配提供依据。方法]以杂交小麦母本晋麦47和父本丰优1号及其F2籽粒为材料，采用SDS—PAGE电泳法对小麦籽粒中的高分子量麦谷蛋白亚基（HMW—GS）的分离及表达进行分析。结果]F2籽粒中共出现21种HMW—GS带型组合，其中回归母本的带型（N、7＋9、3＋12）占69．10％，回归父本的带型（1、17＋18、5＋10）占1．04％，其余29．86％为双亲杂合带型或缺失带型；HMW—GS在F2籽粒中按一定规律进行分离，Glu-Dlb，Glu-Dld出现基因重组（重组率5．90％），17＋18和7＋9亚基同时在Glu—B1位点表达，而Glu—Blc在该位点出现表达空位（空位率5．20％）。结论]明确了HMW—GS在小麦F2籽粒中的表达及分离规律。
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| 关 键 词： | 小麦 F2 HMW-GS 带型 |
Study on Inheritance of HMW-GS in F2 Grain of Jinmai |
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| Institution: | ZHANG Li et al ( College of Arts and Science, Shanxi Agricultural University, Taigu, Shanxi 030801 ) |
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| Abstract: | Objective] The aim was to provide the basis for forecasting processing quality of hybrid wheat and combining parents in high quality wheat breeding process.Method] With grains of female parent Jinmai 47, male parent Fengyou 1 and F2 of hybrid wheat as the materials, the isolation and expression of high molecular weight glutenin subunits (HMW-GS) in grains were analyzed by SDS-PAGE electrophoresis.Result] 21 kinds of band phenotypes of HMW-GS appeared in F2 grains.Among which band phenotypes that regressed to female parent (N, 7+9, 3+12) was 69.10%, and that regressed to male parent (1、17+18、5+10) was 1.04%, the remained 29.86% band phenotypes were hybrid band phenotypes and deletion band phenotypes..HMW-GS isolated in F2 grains according to a certain inheritance, Glu-Dlb and Glu-Dld was recombined (recombination rate of 5.90%), 17+18 and 7+9 subunits expressed at Glu-B1 site at the same time, while there were no expression of Glu-Blc at this site (vacancy rate of 5.20%).Conclusion] This study determined the law of expression and isolation of HMW-GS in F2 grain of wheat. |
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| Keywords: | F2 HMW-GS Wheat F2 HMW-GS Brand phenotypes |
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