| 3种转基因成分检测蛋白芯片的研制 |
| |
| 引用本文： | 汪琳,邢佑尚,周琦,赵胤泽,柏亚铎,蒲静,张伟,乔彩霞.3种转基因成分检测蛋白芯片的研制[J].植物检疫,2011(3). |
| |
| 作者姓名： | 汪琳 邢佑尚 周琦 赵胤泽 柏亚铎 蒲静 张伟 乔彩霞 |
| |
| 作者单位： | 北京出入境检验检疫局;沈阳农业大学; |
| |
| 基金项目： | 国家转基因重大专项课题(2008ZX08012-001) |
| |
| 摘 要： | 为研制一种可同时检测3种转基因成分BTCry1Ac蛋白、植酸酶蛋白、BTCry1Ah蛋白的蛋白芯片。本文将3种转基因成分蛋白质的单克隆抗体点于化学修饰的基片上,对芯片的表面修饰材料、点样缓冲液成分、封闭液种类、标记二抗使用浓度、反应时间进行优化,并对芯片检测的特异性、灵敏度进行测定。结果表明:研制芯片的表面为环氧修饰,使用含0.1%BSA和30%甘油的点样缓冲液和含1%BSA的封闭液,每次反应时间为30min时,有最佳反应结果;其检测灵敏度为:BTCry1Ac蛋白35ng/mL、植酸酶蛋白20ng/mL、BTCry1Ah蛋白30ng/mL,信号稳定;本研究建立的抗体蛋白芯片检测法可同时检测3种转基因蛋白,并具有较高的灵敏性、特异性和可靠性。
|
| 关 键 词： | 蛋白芯片 转基因蛋白 检测 |
Development of protein chip for detection three elements of GMO |
| |
| Wang Ling,Xing Youshang,Zhou Qi,Zhao Yinze,Bai Yaduo,Pu Jing,Zhang Wei,Qiao Caixia.Development of protein chip for detection three elements of GMO[J].Plant Quarantine,2011(3). |
| |
| Authors: | Wang Ling Xing Youshang Zhou Qi Zhao Yinze Bai Yaduo Pu Jing Zhang Wei Qiao Caixia |
| |
| Institution: | Wang Ling1,Xing Youshang1,2,Zhou Qi1,Zhao Yinze1,Bai Yaduo1,Pu Jing1,Zhang Wei1,Qiao Caixia1(1.Beijing Entry-Exit Inspection and Quarantine Bureau,Beijing 100026,China,2.Agricultural University of Shenyang) |
| |
| Abstract: | The protein chip was prepared for detecting the three transgenic proteins: BT Cry1Ac,Phytase and BT Cry1Ah.Three kinds of monoclonal antibodies corresponding to the proteins were dotted on the chemically modified glass slides,superficial treatment process,spotting concentration,blocking solution type,the concentration of labeled secondary antibody and reaction time were optimized.The detection specificity,and sensitivity were verified.Under the following conditions can obtain the optimal result:Surface of t... |
| |
| Keywords: | protein chip transgenic protein detection |
| 本文献已被 CNKI 等数据库收录！ |
|
