A molecular technique for selection of self-compatible varieties of Japanese pear (Pyrus pyrifolia Nakai) |
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| Authors: | Hoy-Taek Kim Yutaka Hirata Yong-Uk Shin Hae-Sung Hwang Jeong-Hwan Hwang Il-Sheob Shin Dae-Il Kim Sang-Jo Kang Hyun-Jung Kim Dong-Young Shin Ill-Sup Nou |
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| Institution: | (1) Laboratory of Plant Breeding and Biotechnology, Graduate School of International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo 138-8509, Japan;(2) Fruit Tree Breeding Division, National Horticultural Research Institute, Korea;(3) Faculty of Plant Science and Production, Sunchon National University, Sunchon 540-742, Korea |
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| Abstract: | The pear cultivar ‘Osa-Nijisseiki’ (S2Ssm
4; sm = stylar-part mutant) has been used as a parent to breed self-compatible cultivars that produce excellent fruits. However,
determination of the self-compatibility of ‘Osa-Nijisseiki’ offspring requires a lot of time, 6 years or more, by conventional
cross breeding. We have designed a rapid reliable method for the identification of self-compatible varieties of ‘Osa-Nijisseiki’
offspring based on the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with S-allele specific
restriction endonucleases. By using this method, 8 self-compatible varieties were selected among 16 selections resulting from
a cross between the self-compatible cultivar ‘Osa-Nijisseiki’ (S2Ssm
4) and the self-incompatible cultivars ‘Niitaka’ (S3S9), ‘Whasan’ (S3S5), ‘Chuwhangbae’ (S4S6). The S-genotypes of 16 ‘Osa-Nijisseiki’ offsprings were also determined.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | gametophytic self-incompatibility PCR-RFLP pear (Pyrus pyrifolia Nakai) self-compatibility S-RNase S-genotype |
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