|
|||||
|
|
Persistent sub-lethal chlorine exposure elicits the temperature induced stress responses in Cyprinus carpio early fingerlings |
|
| Authors: | AK Verma SM Manush RS Dalvi PM Ravi |
| Institution: | a College of Biotechnology, Allahabad Agricultural Institute (Deemed University), Allahabad, Uttar Pradesh 211007, India b Fish Biochemistry Laboratory, Central Institute of Fisheries Education, Fisheries University Road, 7 Bunglows, Versova, Andheri (W), Mumbai 400061, India c Board of Research in Nuclear Sciences, Department of Atomic Energy, India d Environmental Survey Laboratory, Kaiga, Karnataka, India e Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, India |
| Abstract: | Thermal effluents discharged through cooling systems of nuclear power plants often contain chlorine (used to control bio-fouling), which may affect the metabolic status of fishes. In order to evaluate the hypothesis, we tested the effect of high temperature and a persistent sub-lethal chlorine exposure on stress responses in Cyprinus carpio advanced fingerlings. Fishes were acclimated to four different temperatures (26, 31, 33, and 36 °C) and maintained for 30 days in two different groups. Subsequently, one of the groups was exposed to persistent chlorine (0.1 mg L−1) for another 28 days and was compared with their respective temperature controls (without chlorine exposure). Sub-lethal doses of pollutants and increasing temperatures with in the tolerance range may not always register any morphological changes Therefore, we studied organ specific biochemical pathways viz. aspartate amino transferase, alanine amino transferase (enzymes of protein metabolism) in liver and muscle; fructose 1,6 diphosphatase (gluconeogenic pathway), in liver; pyruvate kinase, malate dehydrogenase, and lactate dehydrogenase (glycolytic pathway) in muscle; glucose-6-phosphate dehydrogenase (pentose phosphate pathway) in liver; alkaline phosphatase (phosphorus metabolism) in intestine, liver, and muscle; acetylcholine esterase (neurotransmitting enzyme) in brain, and adenosine triphosphate (for membrane transport) in gills at two different acclimation periods (14 and 28 days). The results indicate that C. carpio fingerlings demonstrated metabolic readjustments with increasing temperatures, in order to cope with energy demand of the cell. However, exposure to chlorine at higher temperatures affected protein metabolism, gluconeogenic pathway and subsequently glycolytic pathway, leading to an energy-limited condition. In addition, alteration of membrane transport and neurotransmission might be an early indication of cellular damage. Overall results indicate that persistent sub-lethal chlorine exposure elicits temperature induced stress response in C. carpio early fingerlings. |
| Keywords: | Cyprinus carpio Acclimation temperatures Chlorine Metabolic enzymes Stress |
| 本文献已被 ScienceDirect 等数据库收录！ | |