Analysis of two acetylcholinesterase genes in Bombyx mori |
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| Authors: | Atsushi Seino An Jang Tan Yoshiaki Kono Takahiro Shiotsuki |
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| Institution: | a Invertebrate Gene Function Research Unit, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan
b Institute of Agriculture and Forestry, Tsukuba University, Tsukuba 305-8572, Japan |
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| Abstract: | Two acetylcholinesterases (AChE, EC 3.1.1.7) cDNAs were identified and cloned from silkworm, Bombyx mori. One of those, BmAChE-o cDNA, is comprised of 3197 nucleotides which encode 638 amino acids, having an amino acid sequence homology of 72% with Drosophila melanogaster Ace-orthologous AChE (AO-AChE). In some species, another AChE group based on the sequence, Drosophila Ace-paralogous AChE (AP-AChE) has been recognized in relation to organophosphate- or carbamate-resistance, but there have been few reports of AP-AChE among lepidopteran species. However, we isolated the AP-AChE from lepidopteran silkworm, and cloned full ORF as BmAChE-p, which cDNA consisted of 2465 nucleotides that encode 683 amino acids. The homologies with other AP-AChEs were over 60% when compared. Although silkworm is not a target of pesticides, the genomic information obtained in this study will contribute to insecticide-resistance study on lepidopteran pest species. |
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| Keywords: | Acetylcholinesterase Ace-orthologous Ace-paralogous Bombyx mori |
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