|
|||||
|
|
| 含CpG序列PRRSV ORF5基因真核表达质粒在小鼠的细胞免疫应答 | |
| 引用本文： | 温建新,李俊,周顺,任慧英,刘文华,邹玲,徐文,牛钟相,王金宝.含CpG序列PRRSV ORF5基因真核表达质粒在小鼠的细胞免疫应答[J].中国兽医学报,2009,29(12). |
| 作者姓名： | 温建新 李俊 周顺 任慧英 刘文华 邹玲 徐文 牛钟相 王金宝 |
| 作者单位： | 1. 山东农业大学,动物科技学院,山东,泰安,271018;青岛农业大学,动物科技学院,山东,青岛,266109 2. 山东省农业科学院,山东,济南,250000 3. 青岛农业大学,动物科技学院,山东,青岛,266109 4. 山东农业大学,动物科技学院,山东,泰安,271018 |
| 摘 要： | 为了提高猪繁殖与呼吸综合征病毒(PRRSV)基因免疫的效果,构建含CpG基序和PRRSV ORF5的真核重组表达质粒CpG-pVAX1-ORF5.经酶切鉴定和序列测定,将重组质粒转染COS-7细胞,经间接免疫荧光试验证实CpG-pVAX1-ORF5可表达PRRSV GP5蛋白.免疫SPF小鼠,检测鼠的脾T淋巴细胞亚群数量(CD4+和CD8+)以及淋巴细胞增殖反应(MTT法)水平.结果表明本试验构建的含CpG基序真核重组表达质粒CpG-pVAX1-ORF5能诱导小鼠产生针对PRRSV的细胞免疫. |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5基因 细胞免疫应答 |
Cellular immune response to eukaryotic expression vector containing PRRSV ORF5 and CpG motifs |
|
| WEN Jian-xin,LI Jun,ZHOU Shun,REN Hui-ying,LIU Wen-hua,ZOU Lin,XU Wen,NIU Zhong-xiang,WANG Jin-bao.Cellular immune response to eukaryotic expression vector containing PRRSV ORF5 and CpG motifs[J].Chinese Journal of Veterinary Science,2009,29(12). | |
| Authors: | WEN Jian-xin LI Jun ZHOU Shun REN Hui-ying LIU Wen-hua ZOU Lin XU Wen NIU Zhong-xiang WANG Jin-bao |
| Abstract: | To improve the gene immunization against porcine reproductive and respiratory syndrome virus(PRRSV),the eukaryotie expression vector CpG-pVAX1-ORF5 containing PRRSV ORF5 gene coding region and CpG motif we constructed After identified by enzyme analysis and nucleotide sequencing test,the recombinant expression vector plasmid CpG-pVAX1-ORF5 was transfected into COS-7 cells.The transient expression of PRRSV ORF5 nucleocapsid protein was detected by indirect immunofluorescence assay(IFA).The recombinant expression plasmid was inoculated into SPF mice.The number of CD4+、CD8+and the lymphocyte proliferation test(MTT)in peripheral blood of the vaccinated mice were used to detect.The results showed that ehd recombinant plasmid CpG-pVAX1-ORF5 could induce higher cellular immune response. |
| Keywords: | porcine reproductive and respiratory syndrome virus(PRRSV) ORF5 gene cellular immune response |
| 本文献已被 万方数据 等数据库收录！ | |