Influence of pH,dispersion methods,and different compounds on adenosine triphosphate recovery from soil |
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| Authors: | Gian Attilio Sacchi Giovanni Valsecchi Anna Farini Sergio Cocucci |
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| Institution: | (1) Departimento di Fisiologia delle Piante Coltivate e Chimica Agraria, Università degli Studi di Milano, I-20133 Milan, Italy |
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| Abstract: | We studied the recovery of ATP from soil. A soil-water suspension was prepared by two different methods (simple stirring or ballottini mill treatment) at different pH levels and in the presence of different chemicals Na2SO4, Na3PO4, Na5P3O10, adenosine, ethylenediaminetetra-acetic acid (EDTA), TRIS]. The ATP recovery was evaluated by adding 3H]-8 ATP to the solution and comparing the values obtained by radioactivity measurements with those obtained by an enzymatic assay. Strongly acidic (pH lower than 1.0) or alkaline (pH 10.0) extractions yielded the best ATP recoveries compared with intermediate pH values. At pH 10.0, the addition of Na3PO4 or Na5P3O10 gave a high level of ATP recovery, 68 and 96%, respectively. No ATP hydrolysis occurred under alkaline extraction conditions. Under acidic extraction conditions, the addition of adenosine, EDTA, Na2SO4, or Na5P3O10 improved ATP recovery but it was never higher than 34%. The results were discussed in terms of the effects of different physical and chemical conditions on cell disruption, ATP stability, ATP interactions with soil components, and ATP solubilization. |
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| Keywords: | Soil ATP extraction Soil ATP adsorption Microbial biomass Ballottini mill Extracting chemicals |
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