| 枣结果枝cDNA文库的构建与部分ESTs分析 |
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| 引用本文： | 孟玉平,曹秋芬,孙海峰,周慧,张春芬.枣结果枝cDNA文库的构建与部分ESTs分析[J].华北农学报,2009,24(5). |
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| 作者姓名： | 孟玉平 曹秋芬 孙海峰 周慧 张春芬 |
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| 作者单位： | 1. 山西省农业生物技术研究中心,山西,太原,030031
2. 山西大学,化学化工学院,山西,太原,030006 |
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| 基金项目： | 山西省自然科学基金项目 |
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| 摘 要： | 用定向克隆法构建了枣(Ziziphus jujuba Mill)生长初期结果枝的部分cDNA文库,获得1 338个阳性克隆,有557个携带cDNA片段,选取469个长度在500 bp以上的进行测序,得到390个有用序列,其中281个ESTs与NCBI中已知功能基因相似性较高(其中重复性ESTs 101个),有68个ESTs是NCBI中有序
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| 关 键 词： | 壶瓶枣 结果枝 cDNA文库 ESTs分析 |
Construction and Partial ESTs Analysis of a cDNA Library for Fruit-bearing Shoot in Ziziphus jujuba |
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| MENG Yu-ping,CAO Qiu-fen,SUN Hai-feng,ZHOU Hui,ZHANG Chun-fen.Construction and Partial ESTs Analysis of a cDNA Library for Fruit-bearing Shoot in Ziziphus jujuba[J].Acta Agriculturae Boreali-Sinica,2009,24(5). |
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| Authors: | MENG Yu-ping CAO Qiu-fen SUN Hai-feng ZHOU Hui ZHANG Chun-fen |
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| Abstract: | By using directional cloning, a cDNA Library in the fruit-bearing shoot of Ziziphus jujuba Mill during the early stages of flower bud differentiation was constructed. In 1 388 positive clones, 557 cDNA inserts were obtained. 469 cDNA inserts, length over 500 bp, were selected and sequenced, 390 useful inserts were obtained at last. In these useful sequences,281 ESTs (among them there were 101 repetitive ESTs) were higher similarity with the known genes of CNBI, and 68 ESTs were unknown protein that its sequence had published, another 41 ESTs were unknown sequence (new gene) . The known genes were classified by the classification way of arabidopsis thaliana genes, the result indicated that there were the basal metabolism gene 46 genes,the protein synthesis and transfer gene including 27 genes,the photosyn-thesis gene including 24 genes, the cytoarchitecture gene including 22 genes, the signal transduction gene including 19 genes,the auxesis gene including 19 genes,the resistance adversity gene including 11 genes,the flower development gene including 6 genes, the membrane traffic gene including 4 genes, and the metal transfer gene including 2 genes. The ex-pression of some genes may be relation to the properties of jujube trees's high resistance to the adverse environment, for example,cold resistance,drought resistance,barrenness tolerance and heavy metal tolerance. |
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| Keywords: | Ziziphus jujuba Mill HUPING Fruit-bearing shoot cDNA library ESTs analysis |
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