| 鳜传染性脾肾坏死病毒核糖核酸酶III基因结构及序列分析 |
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| 引用本文： | 翁少萍.鳜传染性脾肾坏死病毒核糖核酸酶III基因结构及序列分析[J].水产学报,2001,25(6). |
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| 作者姓名： | 翁少萍 |
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| 作者单位： | 中山大学生命科学学院 广东广州510275
(翁少萍，邓敏，何建国，吕玲，何华虹)，中山大学生命科学学院 广东广州510275(龙綮新) |
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| 基金项目： | 广东省自然科学基金资助项目 (990 2 5 5 ) |
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| 摘 要： | 测定了鳜传染性脾肾坏死病毒 (ISKNV)核糖核酸酶III(RNaseIII)基因的核苷酸全序列。该基因完整读码框为 771bp ,GC含量为 5 2 .5 3% ,编码一个长为 2 5 6个氨基酸、分子量为 2 8.9kD的推定蛋白。其上下游序列各有一个TATAbox ,终止子下游有一段回文序列。与其它物种相比 ,ISKNV与虹彩病毒 (包括LCDV - 1和CIV)的核糖核酸酶III基因的氨基酸序列同源性较高 ,与酵母、线虫等物种的相应基因的同源性较低
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| 关 键 词： | 虹彩病毒 传染性脾肾坏死病毒 核糖核酸酶III基因 序列分析 |
| 收稿时间： | 2014/4/14 0:00:00 |
| 修稿时间： | 2014/4/14 0:00:00 |
Characterization of the ribonuclease III gene of infectious spleen and kidney necrosis virus of Siniperca chuatsi |
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| WENG Shao ping.Characterization of the ribonuclease III gene of infectious spleen and kidney necrosis virus of Siniperca chuatsi[J].Journal of Fisheries of China,2001,25(6). |
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| Authors: | WENG Shao ping |
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| Institution: | School of life science,Zhongshan University,Guangzhou 510275, China |
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| Abstract: | Ribonuclease III gene of infectious spleen and kidney necrosis virus(ISKNV)of Siniperca chuatsi had been sequenced. The open reading frame of the gene is 771 nucleotides long, encoding a putative protein of 256 amino acids with 28.9 kD. The 5' and 3' noncoding regions have TATA boxes. A palindrome is contained downstream of the translation stop codon. Compared with the RNase IIIs found in ISKNV and other species, the higher homology among ISKNV and the two iridovirus is present, and ISKNV has lower identity with other species, especially yeast and rhabditida. |
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