Molecular cloning of canine interleukin-31 and its expression in various tissues |
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| Authors: | Takuya Mizuno Satoshi Kanbayashi Takumi Okawa Sadatoshi Maeda Masaru Okuda |
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| Institution: | aLaboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan;bLaboratory of Veterinary Internal Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan |
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| Abstract: | The newly discovered cytokine, interleukin-31 (IL-31), belongs to the short-chain cytokine group. It was reported that transgenic expression of IL-31-induced pruritus, similar to atopic dermatitis, in mice, further, excessive amounts of IL-31 was also expressed in the skin from human patients with atopic dermatitis as compared to that from normal people. In this study, canine IL-31 was molecularly cloned from concanavalin A-stimulated canine peripheral blood mononuclear cells (PBMCs), and its nucleotide sequence was determined. Canine IL-31 contains 4 alpha-helix structures characteristic of the IL-31 family, and the amino acid identity of canine IL-31 with those of human or mouse is 54% and 28%, respectively. Furthermore, we detected low levels of canine IL-31 in the thymus, testis, spleen, and kidneys, but not in the skin of atopic dogs. |
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| Keywords: | Canine Cytokine IL-31 |
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