| 苏云金杆菌CF1263菌株发酵条件优化的研究 |
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| 引用本文： | 顾晓峰,刘云鹏,解春霞,郑华英.苏云金杆菌CF1263菌株发酵条件优化的研究[J].江苏林业科技,2012,39(1):10-13,20. |
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| 作者姓名： | 顾晓峰 刘云鹏 解春霞 郑华英 |
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| 作者单位： | 1. 句容市农业委员会,江苏句容,212400
2. 江苏省林业科学研究院,江苏南京,211153 |
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| 基金项目： | 江苏省农业科技支撑项目，江苏省林业三项工程项目 |
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| 摘 要： | 对高毒BT菌株——CF1263进行液体振荡培养和正交试验结果表明:依据碳源、氮源和无机盐对芽孢产量的影响,对液体培养基进行成分优化组合,在碳源和氮源中分别筛选出最佳因子,淀粉、蛋白胨、牛肉膏、麦麸、酵母粉,从无机盐中筛选出KH2PO4和MgSO4.7H2O。运用以上7个因素进行L18(37)因素3水平的正交试验,从而得出CF1263最优配方为1.5%淀粉、2%蛋白胨、0.2%牛肉膏、0.3%麦麸、2.0%酵母粉、0.1%KH2PO4和0.2%Mg-SO4.7H2O。
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| 关 键 词： | 苏云金杆菌 液体发酵 最优配方 |
Optimization of fermentation conditions of Bacillus thuringiensis Strain CF1263 |
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| GU Xiao-feng,LIU Yun-peng,XIE Chun-xia,ZHENG Hua-ying.Optimization of fermentation conditions of Bacillus thuringiensis Strain CF1263[J].Journal of Jiangsu Forestry Science & Technology,2012,39(1):10-13,20. |
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| Authors: | GU Xiao-feng LIU Yun-peng XIE Chun-xia ZHENG Hua-ying |
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| Institution: | 1.Jurong Agricultural Committee,Jurong 212400,China;2.Jiangsu Academy of Forestry,Nanjing 211153,China) |
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| Abstract: | For spore production,we have obtained the optimizing combination of carbon,nitrogen,and inorganic salts through the liquid fermentation of high toxic strain CF1263.Starch,peptone,beef extract,wheat bran,yeast powder have successfully been selected from different carbon and nitrogen sources,and KH2PO4 and MgSO4·7H2O from different inorganic salts.Using the above seven factors,the optimum medium for spore production was obtained according to orthogonal experiments and the formulation was 1.5% starch,2% peptone and 0.2% beef extract,0.3% wheat bran,2.0% yeast powder,0.1% KH2PO4,0.2% MgSO4·7H2O. |
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| Keywords: | Bacillus thuringiensis Liquid fermentation Optimal formulation |
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