| 紫外诱变热带微藻选育高油脂藻株 |
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| 引用本文： | 胡小文,马帅,付莉莉,谭德冠,孙雪飘,张家明.紫外诱变热带微藻选育高油脂藻株[J].热带农业科学,2011,31(7):25-28. |
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| 作者姓名： | 胡小文 马帅 付莉莉 谭德冠 孙雪飘 张家明 |
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| 作者单位： | 1. 海南大学农学院,海南儋州,571701
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 中央级公益性研究院所基本科研业务费专项“油藻资源收集、鉴定和开发利用”(ITBBZX2008-4-3); 科技部国际合作重点项目“热带生物质能源高效转化及综合利用”(2010DFA62040) |
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| 摘 要： | 为选育高含油量微藻藻种,本试验以优势藻株La4-37为原材料,采用紫外线辐射法对其进行诱变处理,获得了296株藻株。利用尼罗红荧光检测法对获得的藻株进行荧光检测,筛选获得相对含油量最大的诱变藻株M077和M040。通过对诱变株生长及脂荧光强度动态跟踪发现,诱变株生长周期和油脂积累时期基本一样,当达到平稳期时,诱变株油脂积累能力均有较大提高,脂荧光强度分别是原始藻株的6.2倍和1.7倍。
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| 关 键 词： | 微藻 紫外诱变 尼罗红 油脂 |
Breeding of Lipid-rich Microalgae with Tropical Microalgae by Ultraviolet Mutation |
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| HU Xiaowen , MA Shuai FU Lili TAN Denguang SUN Xuepiao ZHANG Jiaming.Breeding of Lipid-rich Microalgae with Tropical Microalgae by Ultraviolet Mutation[J].Chinese Journal of Tropical Agriculture,2011,31(7):25-28. |
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| Authors: | HU Xiaowen MA Shuai FU Lili TAN Denguang SUN Xuepiao ZHANG Jiaming |
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| Institution: | HU Xiaowen 1,2) MA Shuai 2) FU Lili 2) TAN Denguang 2) SUN Xuepiao 2) ZHANG Jiaming 2) (1 College of Agriculture,Hainan University,Danzhou,Hainan 571701,China,2 Institute of Tropical Bioscience and Biotechnology,CATAS,Haikou,Hainan 571101,China) |
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| Abstract: | In order to obtain algal strains with higher oil content,La4-37 was treated with ultraviolet irradiation.296 microalgae were obtained after preliminary screening.Two mutagenic microalgae M077 and M040 with relatively lipid content were isolated through the lipid fluorescence detection and the lipid fluorescence intensity at the same algal density by Nile Red fluorescence staining method.The growth and lipid dynamic fluorescence intensity of mutagenic microalgae was showed that the growth cycle of mutant str... |
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| Keywords: | microalgae ultraviolet mutagenesis Nile Red lipid |
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