Influence of human mutant p27 gene on the biological behaviors of colorectal cancer cells |
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| Authors: | CHEN Jun XU Shao-yong DENG Chang-sheng WANG Jia-ning HUANG Yun-zhang |
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| Institution: | 1.Department of Gastroenterology,Peoples Hospital,Yunyang Medical College,Shiyan 442000,China;2.Department of Gastroenterology,Zhongnan Hospital,Wuhan University,Wuhan 430071,China.E-mail: sycj@sycatv.net |
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| Abstract: | AIM: To observe the influence of human mutant p27 gene (p27mt) on the growth and so as to investigate the function and mechanism of p27mt in gene therapy for colorectal cancer.METHODS: Colorectal cancer cell line SW480 was infected with recombinant replication defective adenovirus Ad-p27mt,and expression of p27mt protein was detected by Western blotting.The inhibitory effect of p27mt on SW480 and cell cycle were determined by flow cytometry,and DNA fragment was analyzed to identify the occurrence of apoptosis.RESULTS: After transfected with Ad-p27mt,p27 protein was highly expressed in SW480 cells.77.96% colorectal cancer cells were blocked in phase G0/G1,while in Ad-LacZ group and blank control group,27.57% and 25.29% cells were blocked in the same phase,respectively.Growth curve showed Ad-p27mt had an obviously inhibitory effect on the growth of SW480 cells.DNA fragment assay demonstrated that p27mt was able to induce the apoptosis of colorectal cancer cells.CONCLUSION: p27mt has an obvious blocking effect on colorectal cancer cell cycle,and most cells are blocked in phase G0/G1.This blockage is related with the growth inhibition and apoptosis induced by p27mt. |
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| Keywords: | Colorectal neoplasms Genes p27mt Apoptosis Cell cycle |
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