Effects of Resveratrol on in vitro Fertilization of Sheep Oocytes |
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| Authors: | NURIBIYAMU·Maimaitituoheti ZHAO Xi AIRIXIATI·Dilixiati ZHANG Bo ABULIZ·Wusiman |
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| Institution: | College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | The purpose of this study was to investigate the effects of resveratrol (RES) at different concentrations (0,0.5,2.0,5.0 μmol/L) on in vitro fertilization (IVF) and antioxidant capacity of ovine oocytes and the secretion of steroid hormones by cumulus cells.Sheep oocytes were fertilized in vitro after maturated in different concentrations of RES for 24 h,and the in vitro maturation (IVM) medium was collected for detecting the enzyme activity of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and the content of monochrome display adapter (MDA).The ELISA method was used to detect the concentration of estradiol (E2) and progesterone (P4).The results showed that when compared to the control group,adding 0.5 μmol/L RES to the IVM medium significantly increased the cleavage rate (P<0.05),but had no significant effect on the fertilization rate and blastocyst rate (P>0.05);5.0 μmol/L RES significantly reduced the fertilization rate,cleavage rate and blastocyst rate (P<0.05),which had an inhibitory effect on embryonic development.Adding 0.5 μmol/L RES to IVM and IVC (in vitro culture,IVC) medium significantly increased the fertilization rate,cleavage rate and blastocyst rate (P<0.05).Compared to the control group,the addition of RES to IVM solution had a certain inhibitory effect on the secretion of E2 by cumulus cells,5.0 μmol/L RES significantly reduced E2 concentration (P<0.05);0.5 μmol/L RES significantly increased the P4 secretion of cumulus cells (P<0.05).0.5 and 2.0 μmol/L RES increased the activity of SOD,GSH-Px and other enzymes,but there was no significant difference when compared with the control group (P>0.05),but significantly reduced MDA content (P<0.05),while 5.0 μmol/L RES significantly reduced the activity of antioxidant enzymes and increased the content of MDA (P>0.05).In conclusion,0.5 μmol/L RES simultaneously added to IVM and IVC medium could enhance the antioxidant capacity of oocytes and concentration of P4,and reduced the content of MDA,thus improved the cleavage rate and blastocyst rate. |
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| Keywords: | sheep oocyte resveratrol in vitro fertilization antioxidase steroid hormone |
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