uclear factor κB in LPS stimulated rat alveolar macrophages promotes TNF-α secretion |
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| Authors: | ZHANG Fang LI Zi-ling SHI Yi ZHAO Ming XIN Xiao-feng QIAN Gui-sheng |
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| Institution: | 1.Department of Respiration Diseases, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing 210002, China;2.Insititute of Respiration Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China. E-mail: zhangfanglab@yahoo.com.cn |
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| Abstract: | AIM: To investigate the activation of nuclear factor κB (NF-κB) induced by lipopolysaccharides (LPS) in rat alveolar macrophages (AMs) and its regulatory role in tumor necrosis factor (TNF-α) secretion. METHODS: The dynamic activity changes of NF-κB induced by LPS were determined with electrophoretic mobility shift assay (EMSA). Antisense oligonucleotides of NF-κB subunit (p65) were transfected into AMs prior to LPS stimulation. The effect of antisense oligonucleotide transfection on expressions of p65 and TNF-α in supernatant were measured with Western blotting and enzyme linked immunosorbent assay (ELISA), respectively.RESULTS: NF-κB activity increased markedly and reached its peak level at 4 h after LPS stimulation. After transfected with antisense oligonucleotides of NF-κB subunit (p65), expression of p65 and TNF-α in supernatant decreased markedly.CONCLUSION: NF-κB activity has a positive effect on regulating secretion of TNF-α in AMs induced by LPS. |
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| Keywords: | Macrophages alveolar NF-kappa B Lipopolysaccharides Tumor necrosis factor |
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