VEGF induces HUVECs to produce extracellular H2O2 and its proliferation role |
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Authors: | QIAN Zhong-qing ZENG Yao-ying WANG Tong LIN Yi JI Yu-hua ZHAO Jing-xian |
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Institution: | The Key Laboratory of Ministry of Education for Tissue Transplantation & Immunology, Jinan University, Guangzhou 510632, China. E-mail: tzengyy@jnu.edu.cn |
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Abstract: | AIM:To study the effect of VEGF on extracellular H2O2 production in HUVECs and the role of H2O2 in the VEGF-induced proliferation. METHODS:HUVECs was stimulated with 500 μg/L VEGF. Products of extracellular H2O2 was detected by H2DCFDA staining. MTT method was used to value the influences of 3×106 U/L catalase and 5-20 mmol/L H2O2 to VEGF function. RESULTS:After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3×106 U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3×106 U/L catalase. The extrinsic H2O2 at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P<0.01). CONCLUSION:These findings indicate that VEGF induces HUVECs to produce extracellular H2O2 and plays role in proliferation, but extrinsic H2O2 restrains VEGF function. |
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Keywords: | Hydrogen peroxide Vascular endothelial cells Vascular endothelial growth factors Catalase |
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